Detailed information of TA system TA035932

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4378322..4379142
• Replicon: chromosome
• Accession: NZ_AP022525
• Organism: Escherichia coli strain THO-003

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: JLF28_RS21500
• Protein ID: WP_001054379.1
• Coordinates: 4378322..4378579 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: JLF28_RS21505
• Protein ID: WP_000123957.1
• Coordinates: 4378591..4379142 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF28_RS21480	4373869..4374849	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
JLF28_RS21485	4375432..4376538	-	1107	Protein_4215	DNA helicase	-
JLF28_RS21490	4376601..4377748	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
JLF28_RS21495	4377781..4377945	+	165	Protein_4217	GNAT family N-acetyltransferase	-
JLF28_RS21500	4378322..4378579	+	258	WP_001054379.1	YjhX family toxin	Toxin
JLF28_RS21505	4378591..4379142	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
JLF28_RS21510	4379194..4379940	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
JLF28_RS21515	4380068..4380328	+	261	WP_000077645.1	hypothetical protein	-
JLF28_RS21520	4380366..4380482	+	117	Protein_4222	VOC family protein	-
JLF28_RS21525	4380727..4381848	+	1122	WP_000010833.1	M42 family metallopeptidase	-
JLF28_RS21530	4381845..4382123	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
JLF28_RS21535	4382135..4383448	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE	4364550..4387363	22813

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T35932 WP_001054379.1 NZ_AP022525:4378322-4378579 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T35932 NZ_AP022525:4378322-4378579 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT35932 WP_000123957.1 NZ_AP022525:4378591-4379142 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT35932 NZ_AP022525:4378591-4379142 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030