Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 289020..289820 Replicon chromosome
Accession NZ_AP022525
Organism Escherichia coli strain THO-003

Toxin (Protein)


Gene name ataT Uniprot ID F4T503
Locus tag JLF28_RS01315 Protein ID WP_000342452.1
Coordinates 289293..289820 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4T504
Locus tag JLF28_RS01310 Protein ID WP_001277107.1
Coordinates 289020..289286 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF28_RS01290 284679..285347 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
JLF28_RS01295 285340..286398 + 1059 WP_001042013.1 permease-like cell division protein FtsX -
JLF28_RS01300 286643..287497 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
JLF28_RS01305 287768..288871 + 1104 WP_001021994.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
JLF28_RS01310 289020..289286 + 267 WP_001277107.1 DUF1778 domain-containing protein Antitoxin
JLF28_RS01315 289293..289820 + 528 WP_000342452.1 GNAT family N-acetyltransferase Toxin
JLF28_RS01320 289817..290200 - 384 WP_000778796.1 aspartate 1-decarboxylase autocleavage activator PanM -
JLF28_RS01325 290623..291732 + 1110 WP_001296485.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
JLF28_RS01330 291780..292706 + 927 WP_001295097.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
JLF28_RS01335 292703..293980 + 1278 WP_000803819.1 branched chain amino acid ABC transporter permease LivM -
JLF28_RS01340 293977..294744 + 768 WP_000082099.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19673.57 Da        Isoelectric Point: 6.6348

>T35911 WP_000342452.1 NZ_AP022525:289293-289820 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Download         Length: 528 bp

>T35911 NZ_AP022525:289293-289820 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9929.38 Da        Isoelectric Point: 10.0863

>AT35911 WP_001277107.1 NZ_AP022525:289020-289286 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT35911 NZ_AP022525:289020-289286 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061K5K9

Antitoxin

Source ID Structure
AlphaFold DB A0A061YQ57

References