Detailed information of TA system TA035910

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 116539..117065
• Replicon: plasmid pSTN0717-73-2
• Accession: NZ_AP022521
• Organism: Enterobacter cloacae strain STN0717-73

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JLF40_RS25625
• Protein ID: WP_000323025.1
• Coordinates: 116778..117065 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JLF40_RS25620
• Protein ID: WP_000534858.1
• Coordinates: 116539..116778 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF40_RS25595	111772..112146	+	375	WP_023327673.1	hypothetical protein	-
-	112206..112300	+	95	NuclAT_0	-	-
-	112206..112300	+	95	NuclAT_0	-	-
-	112206..112300	+	95	NuclAT_0	-	-
-	112206..112300	+	95	NuclAT_0	-	-
JLF40_RS25600	112350..113719	+	1370	WP_085949440.1	IS3-like element ISKpn8 family transposase	-
JLF40_RS25605	113897..115237	+	1341	WP_004196353.1	ISNCY family transposase	-
JLF40_RS25610	115674..116006	-	333	WP_001317460.1	FlxA-like family protein	-
JLF40_RS25615	116209..116514	-	306	WP_001326990.1	hypothetical protein	-
JLF40_RS25620	116539..116778	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JLF40_RS25625	116778..117065	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JLF40_RS25630	117231..118439	+	1209	WP_015386429.1	IS256 family transposase	-
JLF40_RS25635	118448..118618	+	171	WP_200922136.1	type I toxin-antitoxin system Hok family toxin	-
JLF40_RS25640	119492..119785	+	294	WP_023327733.1	hypothetical protein	-
JLF40_RS25645	119802..120623	+	822	WP_023327732.1	DUF945 domain-containing protein	-
JLF40_RS25650	120652..121191	-	540	WP_032636190.1	lytic transglycosylase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..159851	159851
	-	inside	IScluster/Tn	-	-	112350..123436	11086

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T35910 WP_000323025.1 NZ_AP022521:116778-117065 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T35910 NZ_AP022521:116778-117065 [Enterobacter cloacae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT35910 WP_000534858.1 NZ_AP022521:116539-116778 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT35910 NZ_AP022521:116539-116778 [Enterobacter cloacae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8