Detailed information of TA system TA035909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 153947..154473
• Replicon: plasmid pSTN0717-73-1
• Accession: NZ_AP022520
• Organism: Enterobacter cloacae strain STN0717-73

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JLF40_RS24430
• Protein ID: WP_000323025.1
• Coordinates: 154186..154473 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JLF40_RS24425
• Protein ID: WP_000534858.1
• Coordinates: 153947..154186 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF40_RS24395	149437..149823	-	387	Protein_152	NAD-binding protein	-
JLF40_RS24400	149867..150571	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLF40_RS24405	150635..151117	-	483	Protein_154	DNA (cytosine-5-)-methyltransferase	-
JLF40_RS24410	151180..151884	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLF40_RS24415	151989..152561	+	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
JLF40_RS24420	152761..153684	+	924	WP_000167917.1	cation diffusion facilitator family transporter	-
JLF40_RS24425	153947..154186	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JLF40_RS24430	154186..154473	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JLF40_RS24435	154545..154703	+	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
JLF40_RS24440	155312..155632	-	321	WP_000332796.1	hypothetical protein	-
JLF40_RS24445	155914..156117	-	204	WP_001015183.1	hypothetical protein	-
JLF40_RS24450	156164..156514	-	351	WP_000743059.1	hypothetical protein	-
JLF40_RS24455	156574..157173	-	600	WP_001031420.1	hypothetical protein	-
JLF40_RS24460	157272..158216	-	945	WP_000778029.1	DUF5417 domain-containing protein	-
JLF40_RS24465	158727..158903	+	177	WP_001371930.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA2 / qacE / sul1 / qnrA1 / tet(A) / blaSHV-12 / mcr-9 / dfrA16 / catA1	-	1..266476	266476
	-	flank	IS/Tn	-	-	151180..151884	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T35909 WP_000323025.1 NZ_AP022520:154186-154473 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T35909 NZ_AP022520:154186-154473 [Enterobacter cloacae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT35909 WP_000534858.1 NZ_AP022520:153947-154186 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT35909 NZ_AP022520:153947-154186 [Enterobacter cloacae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8