Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4814321..4814937 Replicon chromosome
Accession NZ_AP022519
Organism Enterobacter cloacae strain STN0717-73

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag JLF40_RS23270 Protein ID WP_080277799.1
Coordinates 4814321..4814692 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag JLF40_RS23275 Protein ID WP_033146961.1
Coordinates 4814695..4814937 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF40_RS23255 4811821..4812723 + 903 WP_010436855.1 formate dehydrogenase subunit beta -
JLF40_RS23260 4812720..4813355 + 636 WP_010436852.1 formate dehydrogenase cytochrome b556 subunit -
JLF40_RS23265 4813352..4814281 + 930 WP_023309751.1 formate dehydrogenase accessory protein FdhE -
JLF40_RS23270 4814321..4814692 - 372 WP_080277799.1 type II toxin-antitoxin system VapC family toxin Toxin
JLF40_RS23275 4814695..4814937 - 243 WP_033146961.1 ribbon-helix-helix domain-containing protein Antitoxin
JLF40_RS23280 4815137..4816057 + 921 WP_033146962.1 alpha/beta hydrolase -
JLF40_RS23285 4816066..4817007 - 942 WP_033146963.1 fatty acid biosynthesis protein FabY -
JLF40_RS23290 4817052..4817489 - 438 WP_033146964.1 D-tyrosyl-tRNA(Tyr) deacylase -
JLF40_RS23295 4817486..4818367 - 882 WP_033146965.1 virulence factor BrkB family protein -
JLF40_RS23300 4818361..4818960 - 600 WP_033146966.1 glucose-1-phosphatase -
JLF40_RS23305 4819049..4819519 - 471 WP_033146967.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-109)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-49)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13687.90 Da        Isoelectric Point: 6.9789

>T35907 WP_080277799.1 NZ_AP022519:c4814692-4814321 [Enterobacter cloacae]
MEHMAVFDTNILIDLFNNRVEAADAIDRTASHRAISLITWMEVMVGARKHGHEAKTATVMGVFEIIDITRDIAERSVILR
EKHGMKLPDAIILATAQSRSCPLISRNTKDFLGITGVVSPYQL

Download         Length: 372 bp

>T35907 NZ_AP022519:c4814692-4814321 [Enterobacter cloacae]
ATGGAGCACATGGCGGTGTTCGATACCAATATACTCATCGATCTTTTTAATAATCGCGTTGAGGCTGCGGATGCAATTGA
TCGCACAGCTTCTCATCGAGCCATTAGTCTGATCACATGGATGGAAGTCATGGTGGGGGCCCGCAAACATGGTCACGAAG
CGAAAACTGCGACAGTGATGGGCGTATTTGAGATTATTGATATCACCCGAGATATCGCTGAAAGAAGTGTAATTTTGCGC
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATTCTGGCCACAGCTCAGAGCAGAAGTTGCCCGTTAATCTCACGCAA
TACGAAAGACTTTTTGGGCATCACCGGGGTCGTTTCACCTTACCAGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9469.67 Da        Isoelectric Point: 4.2917

>AT35907 WP_033146961.1 NZ_AP022519:c4814937-4814695 [Enterobacter cloacae]
MILSADLDMGRILIDLSEDVIQRLDNLKQLRNRPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Download         Length: 243 bp

>AT35907 NZ_AP022519:c4814937-4814695 [Enterobacter cloacae]
ATGATTCTTTCCGCGGATCTTGATATGGGCAGAATTCTGATCGATTTGTCTGAAGACGTAATTCAACGACTGGATAATCT
GAAGCAGCTGCGTAACCGGCCTCGTGCCGAATTGCTTCGGGAAGCCATTGAGCAATATCTCGACCAGCAGAGTTCATCGG
TTATCCGCGATGCGCTTGGCTTGTGGGGAAACCAGCAGGAAGATGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References