Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 3318781..3319307 Replicon chromosome
Accession NZ_AP022519
Organism Enterobacter cloacae strain STN0717-73

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag JLF40_RS16065 Protein ID WP_000323025.1
Coordinates 3318781..3319068 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag JLF40_RS16070 Protein ID WP_000534858.1
Coordinates 3319068..3319307 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF40_RS16050 3315357..3316295 - 939 WP_014170919.1 1-phosphofructokinase -
JLF40_RS16055 3316295..3317425 - 1131 WP_033146038.1 fused PTS fructose transporter subunit IIA/HPr protein -
JLF40_RS16060 3317787..3318722 + 936 Protein_3151 MFS transporter -
JLF40_RS16065 3318781..3319068 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
JLF40_RS16070 3319068..3319307 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
JLF40_RS16075 3319332..3319637 + 306 WP_001326990.1 hypothetical protein -
JLF40_RS16080 3319840..3320172 + 333 WP_001317460.1 FlxA-like family protein -
JLF40_RS16085 3320233..3321441 - 1209 WP_015386429.1 IS256 family transposase -
JLF40_RS16090 3321932..3323272 - 1341 WP_004196353.1 ISNCY family transposase -
JLF40_RS16095 3323348..3323608 + 261 Protein_3158 MFS transporter -
JLF40_RS16100 3323605..3323859 - 255 WP_033146563.1 YkgJ family cysteine cluster protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3320233..3321441 1208


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T35904 WP_000323025.1 NZ_AP022519:c3319068-3318781 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T35904 NZ_AP022519:c3319068-3318781 [Enterobacter cloacae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT35904 WP_000534858.1 NZ_AP022519:c3319307-3319068 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT35904 NZ_AP022519:c3319307-3319068 [Enterobacter cloacae]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References