Detailed information of TA system TA035893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1761714..1762511
• Replicon: chromosome
• Accession: NZ_AP022519
• Organism: Enterobacter cloacae strain STN0717-73

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: JLF40_RS08350
• Protein ID: WP_172421424.1
• Coordinates: 1762011..1762511 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: JLF40_RS08345
• Protein ID: WP_008499829.1
• Coordinates: 1761714..1761983 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF40_RS08315	1757341..1757784	-	444	WP_032658095.1	helix-turn-helix transcriptional regulator	-
JLF40_RS08320	1757937..1759178	+	1242	WP_033145159.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
JLF40_RS08325	1759212..1759439	-	228	WP_033145160.1	YccJ family protein	-
JLF40_RS08330	1759460..1760056	-	597	WP_023311076.1	NAD(P)H:quinone oxidoreductase	-
JLF40_RS08335	1760447..1760617	+	171	WP_023311077.1	general stress protein	-
JLF40_RS08340	1760735..1761652	+	918	WP_033145161.1	DMT family transporter	-
JLF40_RS08345	1761714..1761983	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
JLF40_RS08350	1762011..1762511	+	501	WP_172421424.1	GNAT family N-acetyltransferase	Toxin
JLF40_RS08355	1762585..1763907	-	1323	WP_033145163.1	pyrimidine utilization transport protein G	-
JLF40_RS08360	1763929..1764423	-	495	WP_032658088.1	pyrimidine utilization flavin reductase protein F	-
JLF40_RS08365	1764433..1765023	-	591	WP_033145164.1	malonic semialdehyde reductase	-
JLF40_RS08370	1765033..1765833	-	801	WP_033145165.1	pyrimidine utilization protein D	-
JLF40_RS08375	1765841..1766227	-	387	WP_033145166.1	pyrimidine utilization protein C	-
JLF40_RS08380	1766239..1766928	-	690	WP_033145167.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18969.67 Da
• Isoelectric Point: 7.9145

>T35893 WP_172421424.1 NZ_AP022519:1762011-1762511 [Enterobacter cloacae]
MLSEGTDYDFEDFDCGEPSLNAFLAEHLVRQHNGRILRAYLLKERDRPRVLGYYTLSGSCFERAMLPSKTQQRRIPYINV
PSVTLGRLAVDKTLQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALNERAKRFYLKQGFIPLTAENSHSLFFPTKSIE
RLFEQE

Nucleotide

• Length: 501 bp

>T35893 NZ_AP022519:1762011-1762511 [Enterobacter cloacae]
ATGTTATCAGAGGGAACCGATTATGACTTCGAGGATTTTGATTGTGGCGAACCCTCGCTAAATGCCTTCCTTGCTGAACA
TCTGGTGCGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATCGTCCGCGAGTCCTGGGTTATT
ACACTTTATCGGGAAGCTGTTTTGAAAGAGCGATGCTTCCTTCTAAGACTCAGCAGCGCCGAATCCCTTATATCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGGAACAACGCTCGTTGCGCATGC
TATGCGCGTTGTCTACCTGGCTTCACAGGCTGTGGGGGTACACGGAATATTTGTTGATGCCCTTAACGAGCGGGCAAAAC
GATTTTATCTGAAGCAGGGCTTTATCCCGCTGACAGCTGAAAACAGCCATTCTTTGTTCTTCCCTACAAAATCTATCGAA
CGGCTGTTTGAGCAAGAGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT35893 WP_008499829.1 NZ_AP022519:1761714-1761983 [Enterobacter cloacae]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT35893 NZ_AP022519:1761714-1761983 [Enterobacter cloacae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGTC
TCGTATTAAACGAAGCATCATGGAATGCGGTGATGGATGCCATCGACAATCCGCCTGAGCCAAATGAACGCCTGAAACGA
GCAGCAAAACGGCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43