Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1066610..1067230 Replicon chromosome
Accession NZ_AP022519
Organism Enterobacter cloacae strain STN0717-73

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag JLF40_RS05000 Protein ID WP_008499287.1
Coordinates 1066610..1066828 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag JLF40_RS05005 Protein ID WP_008499288.1
Coordinates 1066856..1067230 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF40_RS04970 1062624..1062884 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
JLF40_RS04975 1062887..1063027 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JLF40_RS04980 1063024..1063734 - 711 WP_033144788.1 GNAT family N-acetyltransferase -
JLF40_RS04985 1063836..1065296 + 1461 WP_033144789.1 PLP-dependent aminotransferase family protein -
JLF40_RS04990 1065268..1065735 - 468 WP_023310598.1 hypothetical protein -
JLF40_RS04995 1065853..1066404 - 552 WP_033144790.1 maltose O-acetyltransferase -
JLF40_RS05000 1066610..1066828 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
JLF40_RS05005 1066856..1067230 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
JLF40_RS05010 1067742..1070888 - 3147 WP_029741964.1 multidrug efflux RND transporter permease subunit -
JLF40_RS05015 1070911..1072104 - 1194 WP_029741963.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T35892 WP_008499287.1 NZ_AP022519:c1066828-1066610 [Enterobacter cloacae]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T35892 NZ_AP022519:c1066828-1066610 [Enterobacter cloacae]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGGCGTTGTCAGTCAATTGACACGCTTGAGCGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAAC
TGACAATGAATAAACTGTATGACAAAATCCCTACTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT35892 WP_008499288.1 NZ_AP022519:c1067230-1066856 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT35892 NZ_AP022519:c1067230-1066856 [Enterobacter cloacae]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGACCCAACATCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACGCGCAGGATTTGCAAAAATGGCGTAAATCGGGAAATCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTGAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References