Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 601878..602454 Replicon chromosome
Accession NZ_AP022519
Organism Enterobacter cloacae strain STN0717-73

Toxin (Protein)


Gene name shpA Uniprot ID A0A4R0G892
Locus tag JLF40_RS02800 Protein ID WP_010427219.1
Coordinates 601878..602165 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A411GC49
Locus tag JLF40_RS02805 Protein ID WP_033144548.1
Coordinates 602152..602454 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF40_RS02775 596994..597689 + 696 WP_033144544.1 winged helix-turn-helix domain-containing protein -
JLF40_RS02780 597839..598309 + 471 WP_033144545.1 MarR family transcriptional regulator -
JLF40_RS02785 598306..599373 + 1068 WP_101744430.1 HlyD family secretion protein -
JLF40_RS02790 599363..600439 + 1077 WP_023310256.1 DUF2955 domain-containing protein -
JLF40_RS02795 600436..601707 - 1272 WP_033144547.1 DUF445 domain-containing protein -
JLF40_RS02800 601878..602165 + 288 WP_010427219.1 BrnT family toxin Toxin
JLF40_RS02805 602152..602454 + 303 WP_033144548.1 BrnA antitoxin family protein Antitoxin
JLF40_RS02810 602485..603123 - 639 WP_033144549.1 LysE family translocator -
JLF40_RS02815 603171..603914 - 744 WP_033146481.1 AraC family transcriptional regulator -
JLF40_RS02820 604066..605436 + 1371 WP_033144550.1 NAD-dependent succinate-semialdehyde dehydrogenase -
JLF40_RS02825 605483..605803 - 321 WP_033144551.1 hypothetical protein -
JLF40_RS02830 605803..606348 - 546 WP_023310269.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(32-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11174.64 Da        Isoelectric Point: 6.7609

>T35891 WP_010427219.1 NZ_AP022519:601878-602165 [Enterobacter cloacae]
MPTEFEWDTNKAKSNLIKHGIRFEEAVLVFDDPYHLSLQDRHENGEFRWQTIGLVHGLIVIMVAHTVRFESGDEVIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T35891 NZ_AP022519:601878-602165 [Enterobacter cloacae]
ATGCCAACGGAGTTTGAATGGGATACCAACAAAGCGAAAAGCAATCTCATTAAGCATGGGATCCGCTTTGAAGAAGCTGT
TCTGGTGTTTGACGATCCTTACCATTTATCTTTGCAGGATCGTCATGAAAATGGAGAGTTCCGTTGGCAGACCATTGGCC
TTGTACATGGTCTAATTGTCATTATGGTCGCACACACGGTACGTTTTGAAAGTGGTGACGAAGTCATTCGAATCATTAGC
GCACGAAAGGCGGACCGTAAAGAGAGGAGCCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11395.04 Da        Isoelectric Point: 10.2483

>AT35891 WP_033144548.1 NZ_AP022519:602152-602454 [Enterobacter cloacae]
MSMVKHKRGSAPEMSARREAELKALAEKSEAEIDYSDIPATGDEQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT35891 NZ_AP022519:602152-602454 [Enterobacter cloacae]
ATGAGCATGGTTAAACATAAACGCGGTAGTGCGCCTGAGATGAGTGCTCGCCGTGAGGCTGAGCTCAAAGCACTAGCCGA
AAAATCGGAAGCAGAGATTGATTACAGCGATATCCCTGCCACGGGAGATGAACAGTGGTCAGGGGCGGTGCGAGGGAAGT
TCTATCGGCCACTCAAAACTCAGGCGTCTGTACGTATCGACGCTGACGTGATGGAGTGGCTTAAACGGCCTGGAAAAGGT
TACCAGACGAGGCTCAATGCCATTTTGCGTGAAGCTATGCTGCGCGATCAAAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0G892

Antitoxin

Source ID Structure
AlphaFold DB A0A411GC49

References