Detailed information of TA system TA035888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 256947..257539
• Replicon: chromosome
• Accession: NZ_AP022519
• Organism: Enterobacter cloacae strain STN0717-73

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF40_RS01140
• Protein ID: WP_033144376.1
• Coordinates: 257237..257539 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A808IFF3
• Locus tag: JLF40_RS01135
• Protein ID: WP_033144375.1
• Coordinates: 256947..257234 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF40_RS01130	255319..256950	+	1632	WP_033144374.1	Na/Pi cotransporter family protein	-
JLF40_RS01135	256947..257234	-	288	WP_033144375.1	putative addiction module antidote protein	Antitoxin
JLF40_RS01140	257237..257539	-	303	WP_033144376.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF40_RS01145	257737..258609	+	873	WP_033144377.1	23S rRNA pseudouridine(2604) synthase RluF	-
JLF40_RS01150	258610..258882	-	273	WP_023309994.1	DUF3811 domain-containing protein	-
JLF40_RS01155	258933..259877	-	945	WP_033144378.1	ketopantoate/pantoate/pantothenate transporter PanS	-
JLF40_RS01160	259971..261320	-	1350	WP_033144379.1	lysine-sensitive aspartokinase 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-89)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11284.02 Da
• Isoelectric Point: 9.9325

>T35888 WP_033144376.1 NZ_AP022519:c257539-257237 [Enterobacter cloacae]
MKEIVQTESFQRWEQNLKDRRAKTIIASRLFRLANGLAGDIKPVGEGISELRIHYGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNPNGRSE

Nucleotide

• Length: 303 bp

>T35888 NZ_AP022519:c257539-257237 [Enterobacter cloacae]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCAACGCTGGGAGCAAAACTTAAAAGATCGGCGAGCGAAAACCATTATCGC
TTCCCGCCTCTTTCGGTTGGCAAATGGTTTAGCGGGCGACATTAAACCCGTGGGCGAAGGTATAAGTGAGCTGAGGATCC
ACTATGGTCCGGGCTACAGAATCTATTTTAAAGACCAGGGCAATTGCATCATCGTGCTGTTGTGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATCCCAATGGCAGGAGTGAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10111.59 Da
• Isoelectric Point: 6.4596

>AT35888 WP_033144375.1 NZ_AP022519:c257234-256947 [Enterobacter cloacae]
MHKLTPYDPANALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKHAGD

Nucleotide

• Length: 288 bp

>AT35888 NZ_AP022519:c257234-256947 [Enterobacter cloacae]
ATGCATAAATTAACACCCTACGATCCAGCCAATGCGCTGGTGGATGACGAGGAAATCGCCGTGTTTATGGCTGATGCCTT
AGAAACGGGCGATTCAGCGTATATTGCTAAAGCTCTGGGCGTCATCGCGCGAGCGAAAGGAATGTCGACCATTTCACAGC
AAACTGGCCTATCACGAGAGCAACTGTATCGATCGTTCAGTGATAAAGGAAACCCAACGCTTAAAACCACTCTGGCGGTC
ATGAAAGCATTGGGTCTTGGGCTAACAATCAAACACGCTGGAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A808IFF3