Detailed information of TA system TA035885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4913706..4914322
• Replicon: chromosome
• Accession: NZ_AP022513
• Organism: Citrobacter portucalensis strain STN0717-72

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF48_RS23600
• Protein ID: WP_126957449.1
• Coordinates: 4913706..4914080 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: JLF48_RS23605
• Protein ID: WP_003825279.1
• Coordinates: 4914080..4914322 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF48_RS23585	4911209..4912111	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
JLF48_RS23590	4912108..4912743	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
JLF48_RS23595	4912740..4913669	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
JLF48_RS23600	4913706..4914080	-	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF48_RS23605	4914080..4914322	-	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLF48_RS23610	4914528..4915436	+	909	WP_200920942.1	alpha/beta hydrolase	-
JLF48_RS23615	4915501..4915743	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
JLF48_RS23620	4915736..4916023	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF48_RS23625	4916029..4916970	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
JLF48_RS23630	4917015..4917452	-	438	WP_126957455.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF48_RS23635	4917449..4918321	-	873	WP_008786998.1	virulence factor BrkB family protein	-
JLF48_RS23640	4918315..4918914	-	600	WP_003825293.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T35885 WP_126957449.1 NZ_AP022513:c4914080-4913706 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T35885 NZ_AP022513:c4914080-4913706 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAATATCCTAATCGACCTGTTTAGCGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGTCGCCACAGAACGCAATCAGCCTCATTACATGGATGGAGGTAATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGCACTCGCGTTGCGCTAGGTGCTTTTAACGTGATCAGCATTTCACAGGATATCGCTGAACGAAGCGTGAGTATC
AGGCAGGAACATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGCTTCATCGTCTTACACTGGTTACGCG
TAATACCAAGGATTTTGCAGGCATATCTGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT35885 WP_003825279.1 NZ_AP022513:c4914322-4914080 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT35885 NZ_AP022513:c4914322-4914080 [Citrobacter portucalensis]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAACCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAAGATCAGGCTGAAACAG
CTATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTACGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8