Detailed information of TA system TA035883

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3836986..3837653
• Replicon: chromosome
• Accession: NZ_AP022513
• Organism: Citrobacter portucalensis strain STN0717-72

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: JLF48_RS18515
• Protein ID: WP_200922842.1
• Coordinates: 3837324..3837653 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF48_RS18510
• Protein ID: WP_087934336.1
• Coordinates: 3836986..3837303 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF48_RS18465	3832435..3833256	+	822	WP_200922835.1	DUF945 domain-containing protein	-
JLF48_RS18470	3833473..3834174	+	702	WP_200922836.1	WYL domain-containing protein	-
JLF48_RS18475	3834215..3834451	+	237	WP_200922837.1	hypothetical protein	-
JLF48_RS18480	3834451..3834891	+	441	WP_097344773.1	hypothetical protein	-
JLF48_RS18485	3834915..3835382	+	468	WP_200922838.1	hypothetical protein	-
JLF48_RS18490	3835459..3835698	+	240	WP_087934338.1	DUF905 domain-containing protein	-
JLF48_RS18495	3835798..3836247	+	450	WP_200922839.1	antirestriction protein	-
JLF48_RS18500	3836256..3836738	+	483	WP_200922840.1	DNA repair protein RadC	-
JLF48_RS18505	3836747..3836968	+	222	WP_200922841.1	DUF987 domain-containing protein	-
JLF48_RS18510	3836986..3837303	+	318	WP_087934336.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF48_RS18515	3837324..3837653	+	330	WP_200922842.1	TA system toxin CbtA family protein	Toxin
JLF48_RS18520	3838025..3839257	+	1233	WP_154023425.1	integrase arm-type DNA-binding domain-containing protein	-
JLF48_RS18525	3839342..3841363	+	2022	WP_200924024.1	hypothetical protein	-
JLF48_RS18530	3841553..3842017	-	465	WP_200922843.1	hypothetical protein	-
JLF48_RS18535	3842019..3842255	-	237	WP_200922844.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3817557..3850272	32715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12369.23 Da
• Isoelectric Point: 7.1987

>T35883 WP_200922842.1 NZ_AP022513:3837324-3837653 [Citrobacter portucalensis]
MNTLPATTPQAAKLYLSPVSVWQMLLTYLLEQHYGLTLNDTPFSDETVIQEHIDAGVSLSDAVNFLVEKYGLIRIDRKGF
SWREQTPYISVVDILRARRSTGLLKTNVK

Nucleotide

• Length: 330 bp

>T35883 NZ_AP022513:3837324-3837653 [Citrobacter portucalensis]
ATGAATACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATATCTGTCGCCCGTGTCTGTCTGGCAAATGTTACTGAC
ATACCTGCTGGAACAGCACTATGGCCTGACCCTGAACGACACACCATTCAGTGATGAAACCGTAATACAGGAACACATTG
ATGCTGGAGTATCGCTCAGCGATGCGGTGAACTTTCTGGTAGAAAAATACGGGCTTATCCGTATCGACCGAAAGGGATTT
TCGTGGCGAGAACAAACCCCGTATATTTCCGTAGTGGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11770.30 Da
• Isoelectric Point: 6.4639

>AT35883 WP_087934336.1 NZ_AP022513:3836986-3837303 [Citrobacter portucalensis]
MSNTTWGLQRDITPRLGARLVQEGNRLHYLADRASITGKFSDAECPKLDVVFPDFISQIESMLTTGELNPRHAHCVTLYH
NGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT35883 NZ_AP022513:3836986-3837303 [Citrobacter portucalensis]
ATGAGCAACACCACATGGGGCCTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAG
ATTTTATCAGCCAGATAGAGTCGATGTTGACCACTGGTGAACTGAATCCCCGTCATGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGTCGTCTATTCCACTCAACGCTGA