Detailed information of TA system TA035873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2515459..2516195
• Replicon: chromosome
• Accession: NZ_AP022513
• Organism: Citrobacter portucalensis strain STN0717-72

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JLF48_RS12070
• Protein ID: WP_200920428.1
• Coordinates: 2515459..2515941 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JLF48_RS12075
• Protein ID: WP_008784769.1
• Coordinates: 2515929..2516195 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF48_RS12040	2510466..2510987	+	522	WP_191222609.1	GNAT family N-acetyltransferase	-
JLF48_RS12045	2510997..2511833	-	837	WP_200920429.1	DMT family transporter	-
JLF48_RS12050	2511886..2512263	-	378	WP_003832962.1	hypothetical protein	-
JLF48_RS12055	2512695..2512811	+	117	Protein_2367	mechanosensitive ion channel protein MscS	-
JLF48_RS12060	2513192..2513824	+	633	WP_001567369.1	hypothetical protein	-
JLF48_RS12065	2513853..2515256	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF48_RS12070	2515459..2515941	-	483	WP_200920428.1	GNAT family N-acetyltransferase	Toxin
JLF48_RS12075	2515929..2516195	-	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
JLF48_RS12080	2516349..2518331	-	1983	WP_200920427.1	MBL fold metallo-hydrolase	-
JLF48_RS12085	2518586..2518972	+	387	WP_197384987.1	hypothetical protein	-
JLF48_RS12090	2519089..2519718	-	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
JLF48_RS12095	2519956..2521143	-	1188	WP_200920425.1	outer membrane protein transport protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2510466..2519718	9252
	-	flank	IS/Tn	-	-	2513853..2515256	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17625.46 Da
• Isoelectric Point: 9.5814

>T35873 WP_200920428.1 NZ_AP022513:c2515941-2515459 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T35873 NZ_AP022513:c2515941-2515459 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT35873 WP_008784769.1 NZ_AP022513:c2516195-2515929 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT35873 NZ_AP022513:c2516195-2515929 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA