Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 1648983..1649517 Replicon chromosome
Accession NZ_AP022513
Organism Citrobacter portucalensis strain STN0717-72

Toxin (Protein)


Gene name relE Uniprot ID A0A6P1V8P2
Locus tag JLF48_RS07780 Protein ID WP_000143805.1
Coordinates 1649227..1649517 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A7I6XZR7
Locus tag JLF48_RS07775 Protein ID WP_000104338.1
Coordinates 1648983..1649237 (+) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF48_RS07760 1645647..1646627 + 981 WP_033146059.1 IS5-like element ISKpn26 family transposase -
JLF48_RS07765 1647634..1648029 + 396 WP_032637393.1 hypothetical protein -
JLF48_RS07770 1648116..1648826 + 711 WP_032637325.1 tyrosine-type recombinase/integrase -
JLF48_RS07775 1648983..1649237 + 255 WP_000104338.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
JLF48_RS07780 1649227..1649517 + 291 WP_000143805.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF48_RS07785 1649599..1650546 - 948 WP_023356254.1 IS30 family transposase -
JLF48_RS07790 1650651..1651457 - 807 Protein_1530 glucosyl transferase GtrII family protein -
JLF48_RS07795 1651594..1652574 - 981 WP_200923080.1 IS5 family transposase -
JLF48_RS07800 1652643..1653371 - 729 Protein_1532 IS5-like element ISEc68 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - wbbO / wbbN / glf / wzm 1629970..1659992 30022
- inside IScluster/Tn - - 1643190..1657234 14044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-47)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11463.41 Da        Isoelectric Point: 10.2689

>T35872 WP_000143805.1 NZ_AP022513:1649227-1649517 [Citrobacter portucalensis]
MTFNVEFDERAFKEWNKLEKTIREQFKKKLKKLQQNPYVESARLHGDLAGCFKIKLRASGFRLIYKVIDDEIVIWVVAVG
KREDEKAYETARKRLL

Download         Length: 291 bp

>T35872 NZ_AP022513:1649227-1649517 [Citrobacter portucalensis]
ATGACCTTTAATGTTGAATTTGACGAAAGGGCGTTTAAGGAATGGAATAAGCTCGAAAAGACGATCCGTGAGCAATTCAA
AAAGAAGCTAAAAAAGCTTCAGCAAAATCCTTATGTGGAATCAGCCCGACTGCATGGTGATCTGGCTGGCTGCTTTAAAA
TAAAGCTGAGAGCATCTGGTTTTCGTTTGATTTACAAGGTTATCGATGACGAGATTGTTATATGGGTGGTCGCAGTAGGT
AAACGGGAAGACGAAAAAGCCTATGAAACGGCCCGAAAGCGTCTTTTATAA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9708.04 Da        Isoelectric Point: 4.4247

>AT35872 WP_000104338.1 NZ_AP022513:1648983-1649237 [Citrobacter portucalensis]
MSTIQIRVDDELKKDAYQAFEKLNLSPSDALRLFLRYVAENEKLPFAEVSVMVSEDDEDADILAVVRERLKNPARRIRVN
VDDL

Download         Length: 255 bp

>AT35872 NZ_AP022513:1648983-1649237 [Citrobacter portucalensis]
ATGAGCACAATTCAAATCCGCGTTGATGACGAACTGAAAAAAGACGCCTATCAGGCCTTTGAAAAACTGAATCTGTCGCC
TTCAGACGCACTTCGTCTGTTCCTGCGGTATGTCGCAGAGAACGAAAAACTCCCCTTTGCTGAAGTCTCAGTGATGGTGA
GCGAAGATGATGAAGACGCTGATATTCTGGCAGTGGTTCGGGAAAGACTGAAAAATCCGGCAAGACGGATCAGGGTTAAC
GTTGATGACCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6P1V8P2

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6XZR7

References