Detailed information of TA system TA035866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 191840..192366
• Replicon: plasmid pSTN0717-64-1
• Accession: NZ_AP022511
• Organism: Enterobacter hormaechei strain STN0717-64

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: JLF41_RS24760
• Protein ID: WP_000323025.1
• Coordinates: 191840..192127 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: JLF41_RS24765
• Protein ID: WP_000534858.1
• Coordinates: 192127..192366 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF41_RS24725	187410..187586	-	177	WP_001371930.1	hypothetical protein	-
JLF41_RS24730	188097..189041	+	945	WP_000778029.1	DUF5417 domain-containing protein	-
JLF41_RS24735	189140..189739	+	600	WP_001031420.1	hypothetical protein	-
JLF41_RS24740	189799..190149	+	351	WP_000743059.1	hypothetical protein	-
JLF41_RS24745	190196..190399	+	204	WP_001015183.1	hypothetical protein	-
JLF41_RS24750	190681..191001	+	321	WP_000332796.1	hypothetical protein	-
JLF41_RS24755	191610..191768	-	159	WP_001447866.1	type I toxin-antitoxin system Hok family toxin	-
JLF41_RS24760	191840..192127	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
JLF41_RS24765	192127..192366	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
JLF41_RS24770	192629..193552	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
JLF41_RS24775	193752..194324	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
JLF41_RS24780	194429..195133	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
JLF41_RS24785	195394..195591	+	198	WP_078310596.1	DDE-type integrase/transposase/recombinase	-
JLF41_RS24790	195955..196596	-	642	WP_013851373.1	tetracycline resistance transcriptional repressor TetR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(2'')-Ia / aadA2 / qacE / sul1 / blaCTX-M-9 / mcr-9 / tet(A) / dfrA16 / qnrA1 / catA1	-	1..292755	292755

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T35866 WP_000323025.1 NZ_AP022511:c192127-191840 [Enterobacter hormaechei]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T35866 NZ_AP022511:c192127-191840 [Enterobacter hormaechei]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT35866 WP_000534858.1 NZ_AP022511:c192366-192127 [Enterobacter hormaechei]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT35866 NZ_AP022511:c192366-192127 [Enterobacter hormaechei]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8