Detailed information of TA system TA035849

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 1082705..1083420
• Replicon: chromosome
• Accession: NZ_AP022510
• Organism: Enterobacter hormaechei strain STN0717-64

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8I0UM05
• Locus tag: JLF41_RS05120
• Protein ID: WP_023303149.1
• Coordinates: 1083052..1083420 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A8I0UJV2
• Locus tag: JLF41_RS05115
• Protein ID: WP_023303148.1
• Coordinates: 1082705..1083031 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF41_RS05090	1077739..1079004	+	1266	WP_023303143.1	hypothetical protein	-
JLF41_RS05095	1079313..1079747	+	435	WP_023303144.1	hypothetical protein	-
JLF41_RS05100	1079806..1080645	+	840	WP_023303145.1	hypothetical protein	-
JLF41_RS05105	1081335..1082156	+	822	WP_023303146.1	DUF945 domain-containing protein	-
JLF41_RS05110	1082222..1082692	+	471	WP_023303147.1	DNA repair protein RadC	-
JLF41_RS05115	1082705..1083031	+	327	WP_023303148.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF41_RS05120	1083052..1083420	+	369	WP_023303149.1	TA system toxin CbtA family protein	Toxin
JLF41_RS05125	1083770..1084516	-	747	WP_032647364.1	class I SAM-dependent methyltransferase	-
JLF41_RS05130	1084527..1084784	-	258	WP_003863178.1	YjhX family toxin	-
JLF41_RS05135	1085044..1086981	-	1938	WP_032647366.1	bifunctional diguanylate cyclase/phosphodiesterase	-
JLF41_RS05140	1086999..1087493	-	495	WP_023295996.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1061787..1083420	21633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (7-107)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13727.91 Da
• Isoelectric Point: 8.2822

>T35849 WP_023303149.1 NZ_AP022510:1083052-1083420 [Enterobacter hormaechei]
MKNLPATISRAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGIILADAVNFLVDKYELVRIDRRGF
SSQGQVPYLTVTDILHARRACGLMKSCSYREVSNIVLSHSRQ

Nucleotide

• Length: 369 bp

>T35849 NZ_AP022510:1083052-1083420 [Enterobacter hormaechei]
ATGAAAAATCTACCTGCTACAATTTCGCGGGCGGCGAAGCCCTGCCTGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTCTGCTTGAACAGCACTATGGTCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTAATTAAGGAACATATTG
ATGCTGGGATTATCCTGGCCGATGCAGTCAACTTCCTGGTAGATAAGTACGAACTGGTTCGCATTGATCGCAGAGGATTT
AGCTCACAGGGACAAGTACCATATTTGACCGTGACAGATATCCTTCATGCCAGAAGAGCATGTGGATTAATGAAAAGTTG
CTCTTATCGGGAAGTAAGTAATATTGTTCTTAGTCATTCGCGGCAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12215.04 Da
• Isoelectric Point: 7.0266

>AT35849 WP_023303148.1 NZ_AP022510:1082705-1083031 [Enterobacter hormaechei]
MSLIPAHEWGIKSDIVPRLGVRLVQEGNRLHYLADRASITGKFSDAECLKLDIVFPHFIRQMESMLTTGEMNSRHAHCVT
LYHNGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 327 bp

>AT35849 NZ_AP022510:1082705-1083031 [Enterobacter hormaechei]
ATGTCATTAATACCAGCCCATGAATGGGGGATAAAAAGCGATATTGTTCCACGGCTTGGAGTCAGATTAGTTCAGGAAGG
CAACCGGCTGCACTATCTGGCTGACCGGGCTAGTATCACCGGTAAGTTCAGTGACGCCGAATGCCTGAAGTTGGATATCG
TATTTCCGCATTTTATCCGTCAGATGGAATCGATGCTGACCACTGGTGAAATGAATTCTCGCCATGCCCACTGTGTCACT
CTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGTAGTTGCGGCTACGTATACATCGCCATTTATCCCACTCA
ACGCTAA