Detailed information of TA system TA035848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 727308..727884
• Replicon: chromosome
• Accession: NZ_AP022510
• Organism: Enterobacter hormaechei strain STN0717-64

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A800YKM1
• Locus tag: JLF41_RS03450
• Protein ID: WP_015572580.1
• Coordinates: 727308..727595 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A801DSF4
• Locus tag: JLF41_RS03455
• Protein ID: WP_017694570.1
• Coordinates: 727582..727884 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF41_RS03425	722426..723121	+	696	WP_032647138.1	winged helix-turn-helix domain-containing protein	-
JLF41_RS03430	723271..723741	+	471	WP_032647140.1	MarR family transcriptional regulator	-
JLF41_RS03435	723738..724805	+	1068	WP_026094404.1	HlyD family secretion protein	-
JLF41_RS03440	724795..725871	+	1077	WP_015572578.1	DUF2955 domain-containing protein	-
JLF41_RS03445	725868..727139	-	1272	WP_032647142.1	DUF445 domain-containing protein	-
JLF41_RS03450	727308..727595	+	288	WP_015572580.1	BrnT family toxin	Toxin
JLF41_RS03455	727582..727884	+	303	WP_017694570.1	BrnA antitoxin family protein	Antitoxin
JLF41_RS03460	727913..728551	-	639	WP_032647144.1	LysE family translocator	-
JLF41_RS03465	728590..729342	-	753	WP_015572582.1	AraC family transcriptional regulator	-
JLF41_RS03470	729496..730866	+	1371	WP_032647147.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
JLF41_RS03475	731044..731589	-	546	WP_006810254.1	porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (32-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11296.81 Da
• Isoelectric Point: 7.3587

>T35848 WP_015572580.1 NZ_AP022510:727308-727595 [Enterobacter hormaechei]
MPIEYEWDSNKAKSNLQKHAIRFEDAVLVFDDPCHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGDEIIRIIS
ARKADRKERRRYEHR

Nucleotide

• Length: 288 bp

>T35848 NZ_AP022510:727308-727595 [Enterobacter hormaechei]
ATGCCTATAGAGTATGAATGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGTGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11482.13 Da
• Isoelectric Point: 10.4094

>AT35848 WP_017694570.1 NZ_AP022510:727582-727884 [Enterobacter hormaechei]
MSIVKHKRGSAPEVSARREAELKALADKPDREIDYSDIPATEDDRWAGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT35848 NZ_AP022510:727582-727884 [Enterobacter hormaechei]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGTGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA