Detailed information of TA system TA035846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4542069..4542685
• Replicon: chromosome
• Accession: NZ_AP022508
• Organism: Enterobacter bugandensis strain STN0717-56

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLD64_RS21530
• Protein ID: WP_193031919.1
• Coordinates: 4542069..4542440 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: JLD64_RS21535
• Protein ID: WP_015569912.1
• Coordinates: 4542443..4542685 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLD64_RS21515	4539569..4540471	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
JLD64_RS21520	4540468..4541103	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
JLD64_RS21525	4541100..4542029	+	930	WP_028014872.1	formate dehydrogenase accessory protein FdhE	-
JLD64_RS21530	4542069..4542440	-	372	WP_193031919.1	PIN domain-containing protein	Toxin
JLD64_RS21535	4542443..4542685	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLD64_RS21540	4542884..4543804	+	921	WP_199887042.1	alpha/beta hydrolase	-
JLD64_RS21545	4543813..4544754	-	942	WP_047366783.1	fatty acid biosynthesis protein FabY	-
JLD64_RS21550	4544799..4545236	-	438	WP_008501836.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLD64_RS21555	4545233..4546114	-	882	WP_045260235.1	virulence factor BrkB family protein	-
JLD64_RS21560	4546108..4546707	-	600	WP_045260234.1	glucose-1-phosphatase	-
JLD64_RS21565	4546795..4547265	-	471	WP_028014878.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13706.95 Da
• Isoelectric Point: 7.4764

>T35846 WP_193031919.1 NZ_AP022508:c4542440-4542069 [Enterobacter bugandensis]
MKHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKVFAGIDEVLTPYQV

Nucleotide

• Length: 372 bp

>T35846 NZ_AP022508:c4542440-4542069 [Enterobacter bugandensis]
ATGAAGCACATGGCAGTGTTTGATACCAATATTCTCATTGATCTCTTCAACAACCGTGTTGAAGCTGCGGATGCGATTGA
GCATACCGCGTCGCATCGTGCCATCAGCTTAATTACCTGGATGGAAGTGATGGTGGGCGCTCGCAGGCATGGTCATGAAG
CGAAAACGGCAGCCGTGATGGGCGCTTTTGAAATTATTGATGTGTCTCGGGATATCGCGGAAAGAAGCGTTTTACTACGT
GAGAAGCATGGAATGAAACTGCCTGATGCTATCATCCTGGCTACAGCGCAAAGCAGAAAATGTCCGTTGATTTCTCGCAA
CACGAAAGTTTTTGCGGGTATTGACGAGGTCCTTACGCCTTACCAGGTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT35846 WP_015569912.1 NZ_AP022508:c4542685-4542443 [Enterobacter bugandensis]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT35846 NZ_AP022508:c4542685-4542443 [Enterobacter bugandensis]
ATGAGCGTTTTGGCGGAACTTGATATGGGCAGAATACTTATCGATTTGTCTGATGACGTGATTCAACGTTTGGATAACCT
CAAGCAATTGCGAAATCAACCACGTGCCGAACTGCTTCGCGAAGCGATAGAGCTGTATCTCGACCAGCAGAGTTCTTCTG
TTATCCGAAATGCGCTTGGTCTGTGGGGAGACAGAAAAGAGGACGGGCTTGAGTATGAACGCAAGCTGCGTGAGGAGTGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3