Detailed information of TA system TA035834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 1704677..1705474
• Replicon: chromosome
• Accession: NZ_AP022508
• Organism: Enterobacter bugandensis strain STN0717-56

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: JLD64_RS08065
• Protein ID: WP_172616463.1
• Coordinates: 1704974..1705474 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: W7PG43
• Locus tag: JLD64_RS08060
• Protein ID: WP_008499829.1
• Coordinates: 1704677..1704946 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLD64_RS08030	1700307..1700750	-	444	WP_136486173.1	helix-turn-helix transcriptional regulator	-
JLD64_RS08035	1700903..1702144	+	1242	WP_069732506.1	bifunctional glucose-1-phosphatase/inositol phosphatase	-
JLD64_RS08040	1702178..1702405	-	228	WP_028012649.1	YccJ family protein	-
JLD64_RS08045	1702424..1703020	-	597	WP_041910554.1	NAD(P)H:quinone oxidoreductase	-
JLD64_RS08050	1703411..1703581	+	171	WP_001273664.1	general stress protein	-
JLD64_RS08055	1703698..1704615	+	918	WP_199886405.1	DMT family transporter	-
JLD64_RS08060	1704677..1704946	+	270	WP_008499829.1	DUF1778 domain-containing protein	Antitoxin
JLD64_RS08065	1704974..1705474	+	501	WP_172616463.1	N-acetyltransferase	Toxin
JLD64_RS08070	1705533..1706855	-	1323	WP_199886406.1	pyrimidine utilization transport protein G	-
JLD64_RS08075	1706877..1707371	-	495	WP_028012654.1	pyrimidine utilization flavin reductase protein F	-
JLD64_RS08080	1707381..1707971	-	591	WP_041910551.1	malonic semialdehyde reductase	-
JLD64_RS08085	1707981..1708781	-	801	WP_028012656.1	pyrimidine utilization protein D	-
JLD64_RS08090	1708789..1709175	-	387	WP_008499822.1	pyrimidine utilization protein C	-
JLD64_RS08095	1709187..1709876	-	690	WP_047368263.1	pyrimidine utilization protein B	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18873.63 Da
• Isoelectric Point: 8.4785

>T35834 WP_172616463.1 NZ_AP022508:1704974-1705474 [Enterobacter bugandensis]
MFSEGTDYDFGDFDCGEPSLNAFLREHLVRQHNGRILRAYLLKERDHPRVLGYYTLSGSCFERAMLPSKTQQRRIPYLNV
PSVTLGRLAIHKELQGNEWGTTLVAHAMRVVYLASQAVGVHGIFVDALSEQAKRFYLKLGFIPLAGENSHSLFFPTKAIE
RLFEQA

Nucleotide

• Length: 501 bp

>T35834 NZ_AP022508:1704974-1705474 [Enterobacter bugandensis]
ATGTTTTCAGAGGGAACCGACTATGATTTCGGGGATTTTGATTGTGGTGAACCCTCGCTAAATGCCTTCCTGAGAGAACA
CCTGGTACGCCAGCACAATGGACGTATTCTACGAGCCTACCTGCTTAAAGAGCGCGACCATCCGCGCGTGCTGGGCTATT
ACACGCTATCGGGAAGTTGCTTTGAGAGAGCGATGCTTCCTTCTAAGACCCAGCAGCGTCGGATCCCTTATCTCAACGTA
CCCAGCGTGACGCTGGGACGTCTGGCTATTCATAAAGAACTACAGGGAAACGAGTGGGGAACAACGCTTGTTGCACACGC
TATGCGGGTGGTTTACCTGGCGTCGCAGGCTGTGGGAGTACATGGAATATTTGTTGATGCCCTTAGCGAGCAGGCTAAAC
GATTTTATCTGAAGCTGGGGTTCATCCCGCTGGCAGGCGAAAACAGCCATTCTTTGTTCTTCCCGACAAAAGCTATCGAA
CGACTGTTTGAGCAGGCGTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10262.72 Da
• Isoelectric Point: 5.8836

>AT35834 WP_008499829.1 NZ_AP022508:1704677-1704946 [Enterobacter bugandensis]
MPALKKQRIDLRLTDEDKTMIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLVLNEASWNAVMDAIDNPPEPNERLKR
AAKRLRNME

Nucleotide

• Length: 270 bp

>AT35834 NZ_AP022508:1704677-1704946 [Enterobacter bugandensis]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAATCAAACGATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATCGAGCAGCACCGTCGTC
TCGTGTTAAACGAAGCCTCCTGGAATGCGGTGATGGATGCTATCGACAATCCGCCAGAGCCAAATGAACGCCTCAAGCGA
GCGGCAAAACGTCTTCGAAACATGGAGTGA

Structures

Antitoxin

• AlphaFold DB: W7PG43