Detailed information of TA system TA035833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1106075..1106695
• Replicon: chromosome
• Accession: NZ_AP022508
• Organism: Enterobacter bugandensis strain STN0717-56

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A1H8SI38
• Locus tag: JLD64_RS05225
• Protein ID: WP_008499287.1
• Coordinates: 1106075..1106293 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8E1RNX1
• Locus tag: JLD64_RS05230
• Protein ID: WP_028012188.1
• Coordinates: 1106321..1106695 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLD64_RS05195	1102086..1102346	+	261	WP_006176933.1	type B 50S ribosomal protein L31	-
JLD64_RS05200	1102349..1102489	+	141	WP_003859006.1	type B 50S ribosomal protein L36	-
JLD64_RS05205	1102486..1103196	-	711	WP_059357441.1	GNAT family N-acetyltransferase	-
JLD64_RS05210	1103298..1104758	+	1461	WP_069733270.1	PLP-dependent aminotransferase family protein	-
JLD64_RS05215	1104730..1105197	-	468	WP_199887079.1	hypothetical protein	-
JLD64_RS05220	1105314..1105865	-	552	WP_028012187.1	maltose O-acetyltransferase	-
JLD64_RS05225	1106075..1106293	-	219	WP_008499287.1	hemolysin expression modulator Hha	Toxin
JLD64_RS05230	1106321..1106695	-	375	WP_028012188.1	Hha toxicity modulator TomB	Antitoxin
JLD64_RS05235	1107205..1110351	-	3147	WP_047367620.1	multidrug efflux RND transporter permease subunit AcrB	-
JLD64_RS05240	1110374..1111567	-	1194	WP_028012190.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8612.00 Da
• Isoelectric Point: 8.9008

>T35833 WP_008499287.1 NZ_AP022508:c1106293-1106075 [Enterobacter bugandensis]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Nucleotide

• Length: 219 bp

>T35833 NZ_AP022508:c1106293-1106075 [Enterobacter bugandensis]
ATGTCTGATAAACCACTCACAAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTTGAACGCGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCCGCTGACCATCGCCTTGCAGAGC
TAACGATGAATAAGCTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14511.30 Da
• Isoelectric Point: 4.8886

>AT35833 WP_028012188.1 NZ_AP022508:c1106695-1106321 [Enterobacter bugandensis]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Nucleotide

• Length: 375 bp

>AT35833 NZ_AP022508:c1106695-1106321 [Enterobacter bugandensis]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGCAACCATGGCTGGGTAAATGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGTTACGGCATTAACACTCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGTCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1H8SI38