Detailed information of TA system TA035830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 246236..247137
• Replicon: plasmid pSTN0717-53-1
• Accession: NZ_AP022499
• Organism: Enterobacter kobei strain STN0717-53

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: JLH17_RS24725
• Protein ID: WP_008786589.1
• Coordinates: 246236..246694 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: JLH17_RS24730
• Protein ID: WP_008786588.1
• Coordinates: 246691..247137 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH17_RS24680	241962..242204	+	243	WP_007372291.1	hypothetical protein	-
JLH17_RS24685	242215..242613	+	399	WP_200928189.1	hypothetical protein	-
JLH17_RS24690	242697..243023	+	327	WP_007372293.1	hypothetical protein	-
JLH17_RS24695	243386..243724	-	339	WP_007372294.1	hypothetical protein	-
JLH17_RS24700	243962..244405	+	444	WP_007372295.1	hypothetical protein	-
JLH17_RS24705	244524..244865	+	342	WP_007372296.1	hypothetical protein	-
JLH17_RS24710	245024..245578	+	555	WP_007372297.1	hypothetical protein	-
JLH17_RS24715	245628..245894	+	267	WP_007372298.1	hypothetical protein	-
JLH17_RS24720	245962..246192	-	231	WP_007372299.1	hypothetical protein	-
JLH17_RS24725	246236..246694	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
JLH17_RS24730	246691..247137	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
JLH17_RS24735	247286..247603	+	318	WP_016241555.1	hypothetical protein	-
JLH17_RS24740	247645..248487	+	843	WP_007372303.1	hypothetical protein	-
JLH17_RS24745	248459..248737	+	279	WP_007372304.1	hypothetical protein	-
JLH17_RS24750	248797..249240	+	444	WP_007372305.1	hypothetical protein	-
JLH17_RS24755	249527..249691	+	165	WP_007372306.1	hypothetical protein	-
JLH17_RS24760	250259..251101	+	843	WP_007372307.1	HNH endonuclease	-
JLH17_RS24765	251523..251774	+	252	WP_024196080.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..282901	282901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T35830 WP_008786589.1 NZ_AP022499:c246694-246236 [Enterobacter kobei]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T35830 NZ_AP022499:c246694-246236 [Enterobacter kobei]
GTGATCCTTTACCGACTGACCAAGACCCGTTATCTGATGACTGCGTGGTCTGGTCTGGGTGCGAAAGAAACGGGGGGCCG
CTGGAACAGCGTAGGCACTGCAATGGTCTACCTGTCTGAAACGGCCAGCCTTACCATGCTTGAAACGCTGGTACACATTC
ACGCGCCGCAGTTACTGGACGACTTTACACTGCTGAGTCTTGATGTACCGGATGATCAGATCCAGACCTTCGACATGAGC
CGTCTCCCTGATAACTGGGCGAGTGAGGATGCTCCTGCTGAGCTGGCGCTCTACGGTGATAACTGGGCTGAAAGCGGTTC
TTCTATCGCGCTGCGTATTCCAAGTGCGTTGTCTCCGGTTGAATTTAACTACTTGCTGAATCCGGCACACCCTGAACTCT
TTGACCTGATGGCTACGGTTAAGAAAATTCCGTTCCGGTTTGATTCCCGCCTGAAATAA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT35830 WP_008786588.1 NZ_AP022499:c247137-246691 [Enterobacter kobei]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT35830 NZ_AP022499:c247137-246691 [Enterobacter kobei]
ATGACGATGAAAGTTTTTTCTCCAGACGTTACCAAAACGAATCAGCACTGCTTATGGCAGGTTGCTGGGCTGGATAATGA
CGGTATCGCGCTGATGGATAAAATCAATCACGGTCTCGATGGCACAGTAGCTAAGCACATTTCTGAATGGGCTAACATCA
CGCCGTCTGAGCTGCGCAAAATGTCGGGTATACCTAATACGACGTTTAATCGTAGCATCAAAGACCGTTTCACTGCCGAT
CAGAGCGAACGTCTGGTGCGCATTATCCGCGTAATTGAACGCGCTGTAGAGCTGTTTGAAGGTGACAAAGAAGCGGCGCA
CAAATGGCTGAACGAAGCGAACCGTGGTCTGAGCTGGAAATCACCGGCAGAGCTTGTTTCTTCTGAGACAGGTGCGCTTG
AAGTGATGCGCCTGATTACGCGTATTGAGCACGGGGTATACTCGTGA

Structures

Toxin

• AlphaFold DB: S1EZ83