Detailed information of TA system TA035829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 238937..239487
• Replicon: plasmid pSTN0717-53-1
• Accession: NZ_AP022499
• Organism: Enterobacter kobei strain STN0717-53

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: JLH17_RS24655
• Protein ID: WP_007372286.1
• Coordinates: 239179..239487 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: JLH17_RS24650
• Protein ID: WP_007372285.1
• Coordinates: 238937..239176 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH17_RS24605	233944..234339	+	396	WP_024196081.1	hypothetical protein	-
JLH17_RS24610	234521..234712	+	192	WP_016241560.1	hypothetical protein	-
JLH17_RS24615	234843..235538	+	696	WP_007372278.1	hypothetical protein	-
JLH17_RS24620	235561..235758	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
JLH17_RS24625	235811..236269	+	459	WP_008786599.1	hypothetical protein	-
JLH17_RS24630	236417..236557	+	141	WP_007372281.1	hypothetical protein	-
JLH17_RS24635	236573..237205	+	633	WP_007372282.1	hypothetical protein	-
JLH17_RS24640	237666..238337	+	672	WP_007372283.1	hypothetical protein	-
JLH17_RS24645	238334..238798	+	465	WP_008786596.1	hypothetical protein	-
JLH17_RS24650	238937..239176	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
JLH17_RS24655	239179..239487	+	309	WP_007372286.1	CcdB family protein	Toxin
JLH17_RS24660	239508..239666	+	159	WP_016241558.1	hypothetical protein	-
JLH17_RS24665	239821..240375	+	555	WP_007372288.1	hypothetical protein	-
JLH17_RS24670	240445..241062	+	618	WP_007372289.1	hypothetical protein	-
JLH17_RS24675	241145..241615	+	471	WP_007372290.1	hypothetical protein	-
JLH17_RS24680	241962..242204	+	243	WP_007372291.1	hypothetical protein	-
JLH17_RS24685	242215..242613	+	399	WP_200928189.1	hypothetical protein	-
JLH17_RS24690	242697..243023	+	327	WP_007372293.1	hypothetical protein	-
JLH17_RS24695	243386..243724	-	339	WP_007372294.1	hypothetical protein	-
JLH17_RS24700	243962..244405	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..282901	282901

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T35829 WP_007372286.1 NZ_AP022499:239179-239487 [Enterobacter kobei]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T35829 NZ_AP022499:239179-239487 [Enterobacter kobei]
ATGCAATATACCGTTTACCGCAATCCCGGCAACAGCCAGGCATACCCTTACCTGCTGGATATACAGAGCGACATCATTGG
CGAGTTAAATACCCGCCTGGTGATCCCCCTTCATCGGCTTAAAAAAGGAGCCTCGGCCCCGGTTGCGCGATTAACTCCGG
TCATACAGGTGGAAGGGAATGACGTTATCCTGATGACGCATGAAATGGCTTCAGTCCGCGTGAAGCAGCTCGGTCAGGCG
GTCATGGACGCATCTCCGTTCCGGCACACTATCAAAAGTGCGGTGGACTTCCTGCTGGATGGTTTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT35829 WP_007372285.1 NZ_AP022499:238937-239176 [Enterobacter kobei]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT35829 NZ_AP022499:238937-239176 [Enterobacter kobei]
ATGCAGGCTACAGCTGCTCGCCAGAAAAAAACCGTCAGTGTCACCCTTGAGCCAATGCTCGTACAGCAGGCAAAAGACGC
TGGGATTAACTTGTCCGCCACGCTGGCAGAAGCGCTTCAGAGCAAACTGAAGGCCAGTGCAGCGGAAGAATGGAAACGAC
AGAATCGTGCTGGTATTCAGGAGTTGAACCGCATCACTGAAGAGCATGGGTTACTGTCTGACGAATACAGGACGTTCTGA

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3