Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1119824..1120444 Replicon chromosome
Accession NZ_AP022498
Organism Enterobacter kobei strain STN0717-53

Toxin (Protein)


Gene name Hha Uniprot ID A0A2W0G488
Locus tag JLH17_RS05305 Protein ID WP_014882802.1
Coordinates 1119824..1120042 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag JLH17_RS05310 Protein ID WP_008499288.1
Coordinates 1120070..1120444 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLH17_RS05275 1115833..1116093 + 261 WP_010428154.1 type B 50S ribosomal protein L31 -
JLH17_RS05280 1116096..1116236 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JLH17_RS05285 1116233..1116943 - 711 WP_045269151.1 GNAT family N-acetyltransferase -
JLH17_RS05290 1117045..1118505 + 1461 WP_045269150.1 PLP-dependent aminotransferase family protein -
JLH17_RS05295 1118477..1118944 - 468 WP_014882800.1 membrane protein -
JLH17_RS05300 1119063..1119614 - 552 WP_014882801.1 maltose O-acetyltransferase -
JLH17_RS05305 1119824..1120042 - 219 WP_014882802.1 hemolysin expression modulator Hha Toxin
JLH17_RS05310 1120070..1120444 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
JLH17_RS05315 1120956..1124102 - 3147 WP_014882803.1 multidrug efflux RND transporter permease subunit -
JLH17_RS05320 1124125..1125318 - 1194 WP_014882804.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T35817 WP_014882802.1 NZ_AP022498:c1120042-1119824 [Enterobacter kobei]
MSEKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T35817 NZ_AP022498:c1120042-1119824 [Enterobacter kobei]
ATGTCTGAAAAACCACTCACAAAGGTCGATTATTTGATGCGCTTACGTCGTTGTCAGTCAATTGACACCCTCGAACGTGT
CATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCTGACCATCGCCTCGCTGAGC
TAACGATGAATAAGTTATATGACAAAATCCCCACATCCGTGTGGAAATTTGTTCGTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT35817 WP_008499288.1 NZ_AP022498:c1120444-1120070 [Enterobacter kobei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT35817 NZ_AP022498:c1120444-1120070 [Enterobacter kobei]
ATGGACGAGTACTCGCCAAAACGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCATGGCTGGGTAAACGATCCAACGTCTGCCATCAATTTACAGTTGAACGAGCTTATCGAGC
ATATCGCTACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCTCAGGATTTGCAAAAATGGCGCAAATCGGGAAACCGACT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCCGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W0G488

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References