Detailed information of TA system TA035813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 13172..13646
• Replicon: plasmid pSTN0717-36-2
• Accession: NZ_AP022496
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: S1DZ70
• Locus tag: JLF47_RS25035
• Protein ID: WP_000262326.1
• Coordinates: 13172..13438 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: JLF47_RS25040
• Protein ID: WP_000879771.1
• Coordinates: 13422..13646 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS25000	8368..8697	+	330	WP_052909321.1	hypothetical protein	-
JLF47_RS25005	8817..10220	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF47_RS25010	10249..10881	-	633	WP_001567369.1	hypothetical protein	-
JLF47_RS25015	11051..11314	+	264	WP_113416113.1	ISAs1 family transposase	-
JLF47_RS25020	11509..12528	+	1020	WP_057699135.1	acyltransferase	-
JLF47_RS25025	12570..12668	+	99	WP_077785049.1	hypothetical protein	-
JLF47_RS25030	12856..13125	+	270	WP_039062313.1	hypothetical protein	-
JLF47_RS25035	13172..13438	-	267	WP_000262326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF47_RS25040	13422..13646	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
JLF47_RS25045	14553..14753	-	201	WP_023139298.1	antitoxin VbhA family protein	-
JLF47_RS25050	15574..16578	-	1005	WP_000427614.1	IS110-like element IS5075 family transposase	-
JLF47_RS25055	16643..17170	-	528	WP_044258217.1	hypothetical protein	-
JLF47_RS25060	17199..17909	-	711	WP_004393997.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	wbtL	1..118786	118786

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10385.00 Da
• Isoelectric Point: 9.5211

>T35813 WP_000262326.1 NZ_AP022496:c13438-13172 [Citrobacter portucalensis]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRILCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T35813 NZ_AP022496:c13438-13172 [Citrobacter portucalensis]
TTGGTCTGGACGATTAACTATTCCGACCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGGATTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCATTCTGTGTGAGATCCGGGATGACGAGCTGGTTATCCTTGCCGCCACCATT
GGCCATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT35813 WP_000879771.1 NZ_AP022496:c13646-13422 [Citrobacter portucalensis]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT35813 NZ_AP022496:c13646-13422 [Citrobacter portucalensis]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
CTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAAGATTATTATCTTTCAGCAGAAACTGCCGCACGCGTTC
GCCGTGGTGATGAAGCCGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: S1DZ70

Antitoxin

• AlphaFold DB: S1EJF5