Detailed information of TA system TA035811

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4801424..4802040
• Replicon: chromosome
• Accession: NZ_AP022494
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF47_RS22870
• Protein ID: WP_126957449.1
• Coordinates: 4801424..4801798 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: JLF47_RS22875
• Protein ID: WP_003825279.1
• Coordinates: 4801798..4802040 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS22855	4798927..4799829	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
JLF47_RS22860	4799826..4800461	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
JLF47_RS22865	4800458..4801387	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
JLF47_RS22870	4801424..4801798	-	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF47_RS22875	4801798..4802040	-	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLF47_RS22880	4802246..4803154	+	909	WP_200920942.1	alpha/beta hydrolase	-
JLF47_RS22885	4803219..4803461	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
JLF47_RS22890	4803454..4803741	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF47_RS22895	4803747..4804688	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
JLF47_RS22900	4804733..4805170	-	438	WP_126957455.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF47_RS22905	4805167..4806039	-	873	WP_008786998.1	virulence factor BrkB family protein	-
JLF47_RS22910	4806033..4806632	-	600	WP_003825293.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T35811 WP_126957449.1 NZ_AP022494:c4801798-4801424 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T35811 NZ_AP022494:c4801798-4801424 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAATATCCTAATCGACCTGTTTAGCGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGTCGCCACAGAACGCAATCAGCCTCATTACATGGATGGAGGTAATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGCACTCGCGTTGCGCTAGGTGCTTTTAACGTGATCAGCATTTCACAGGATATCGCTGAACGAAGCGTGAGTATC
AGGCAGGAACATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGCTTCATCGTCTTACACTGGTTACGCG
TAATACCAAGGATTTTGCAGGCATATCTGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT35811 WP_003825279.1 NZ_AP022494:c4802040-4801798 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT35811 NZ_AP022494:c4802040-4801798 [Citrobacter portucalensis]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAACCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAAGATCAGGCTGAAACAG
CTATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTACGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8