Detailed information of TA system TA035810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4646407..4646995
• Replicon: chromosome
• Accession: NZ_AP022494
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF47_RS22160
• Protein ID: WP_063941209.1
• Coordinates: 4646407..4646724 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: JLF47_RS22165
• Protein ID: WP_032942057.1
• Coordinates: 4646717..4646995 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS22135	4642175..4642999	+	825	WP_200920925.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JLF47_RS22140	4643068..4644291	+	1224	WP_200920926.1	zinc-binding dehydrogenase	-
JLF47_RS22145	4644293..4645105	+	813	WP_063941212.1	shikimate 5-dehydrogenase	-
JLF47_RS22150	4645155..4645460	-	306	WP_032934126.1	hypothetical protein	-
JLF47_RS22155	4645592..4646143	+	552	WP_200920927.1	AAA family ATPase	-
JLF47_RS22160	4646407..4646724	+	318	WP_063941209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF47_RS22165	4646717..4646995	+	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
JLF47_RS22170	4647106..4647795	+	690	WP_200921622.1	dipeptidase PepE	-
JLF47_RS22175	4647910..4649541	-	1632	WP_003826514.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4636397..4646995	10598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 10.1413

>T35810 WP_063941209.1 NZ_AP022494:4646407-4646724 [Citrobacter portucalensis]
IMEILQTMVFRRWEQNLRDRRAKTLIATRLFRLANGLSGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARSLDYQEVFRHE

Nucleotide

• Length: 318 bp

>T35810 NZ_AP022494:4646407-4646724 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTTTGGCTAATGGTTTATCTGGTGATGTAAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTTGCCCGTTCACTGGATTATCAGGAGGTCTTCCGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT35810 WP_032942057.1 NZ_AP022494:4646717-4646995 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT35810 NZ_AP022494:4646717-4646995 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGTGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTGGCTCGTGCTAAGGGTATGGCGAACATCGCAGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACACTTGCGGTG
ATGAAGGTGCTCGGTATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7