Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3694036..3694656 Replicon chromosome
Accession NZ_AP022494
Organism Citrobacter portucalensis strain STN0717-36

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag JLF47_RS17725 Protein ID WP_002892050.1
Coordinates 3694438..3694656 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag JLF47_RS17720 Protein ID WP_003021733.1
Coordinates 3694036..3694410 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF47_RS17710 3689182..3690375 + 1194 WP_003831044.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLF47_RS17715 3690398..3693547 + 3150 WP_193135115.1 efflux RND transporter permease AcrB -
JLF47_RS17720 3694036..3694410 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
JLF47_RS17725 3694438..3694656 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
JLF47_RS17730 3694838..3695389 + 552 WP_003835924.1 maltose O-acetyltransferase -
JLF47_RS17735 3695506..3695976 + 471 WP_003021724.1 YlaC family protein -
JLF47_RS17740 3696055..3696195 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
JLF47_RS17745 3696197..3696457 - 261 WP_008783961.1 type B 50S ribosomal protein L31 -
JLF47_RS17750 3696646..3698199 + 1554 WP_200920716.1 EAL domain-containing protein -
JLF47_RS17755 3698251..3698604 - 354 WP_003831033.1 DUF1428 family protein -
JLF47_RS17760 3698669..3699298 - 630 WP_003831032.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35808 WP_002892050.1 NZ_AP022494:3694438-3694656 [Citrobacter portucalensis]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T35808 NZ_AP022494:3694438-3694656 [Citrobacter portucalensis]
ATGTCCGATAAACCATTAACTAAAACAGATTATTTGATGCGCCTGCGACGTTGCCAGACAATTGATACGCTAGAGCGCGT
TATTGAAAAAAATAAATATGAATTGTCCGACAACGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACCATGAATAAGCTTTACGACAAGATTCCAACTTCTGTTTGGAAATTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT35808 WP_003021733.1 NZ_AP022494:3694036-3694410 [Citrobacter portucalensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT35808 NZ_AP022494:3694036-3694410 [Citrobacter portucalensis]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAATTTCTCTGCGAAACTCTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGTAACCATGGCTGGGTTAATGACCCTACCTCTGCAACCAGCTTGCAGCTTAATGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATCGCGCAGATAGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGTATTAATACGCAGGATCTGCAGAAATGGCGGAAGTCAGGTAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References