Detailed information of TA system TA035807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2592297..2593033
• Replicon: chromosome
• Accession: NZ_AP022494
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JLF47_RS12375
• Protein ID: WP_200920428.1
• Coordinates: 2592551..2593033 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JLF47_RS12370
• Protein ID: WP_008784769.1
• Coordinates: 2592297..2592563 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS12350	2587349..2588536	+	1188	WP_200920425.1	outer membrane protein transport protein	-
JLF47_RS12355	2588774..2589403	+	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
JLF47_RS12360	2589520..2589906	-	387	WP_197384987.1	hypothetical protein	-
JLF47_RS12365	2590161..2592143	+	1983	WP_200920427.1	MBL fold metallo-hydrolase	-
JLF47_RS12370	2592297..2592563	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
JLF47_RS12375	2592551..2593033	+	483	WP_200920428.1	GNAT family N-acetyltransferase	Toxin
JLF47_RS12380	2593398..2593514	-	117	Protein_2432	mechanosensitive ion channel protein MscS	-
JLF47_RS12385	2593946..2594323	+	378	WP_003832962.1	hypothetical protein	-
JLF47_RS12390	2594376..2595212	+	837	WP_200920429.1	DMT family transporter	-
JLF47_RS12395	2595222..2595743	-	522	WP_191222609.1	GNAT family N-acetyltransferase	-
JLF47_RS12400	2595854..2596612	+	759	WP_126318110.1	trans-aconitate 2-methyltransferase	-
JLF47_RS12405	2596744..2597121	+	378	WP_003832953.1	GFA family protein	-
JLF47_RS12410	2597118..2598032	-	915	WP_200920430.1	bestrophin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17625.46 Da
• Isoelectric Point: 9.5814

>T35807 WP_200920428.1 NZ_AP022494:2592551-2593033 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T35807 NZ_AP022494:2592551-2593033 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT35807 WP_008784769.1 NZ_AP022494:2592297-2592563 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT35807 NZ_AP022494:2592297-2592563 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA