Detailed information of TA system TA035806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2485841..2486480
• Replicon: chromosome
• Accession: NZ_AP022494
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: JLF47_RS11920
• Protein ID: WP_200921558.1
• Coordinates: 2485841..2486038 (+)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JLF47_RS11925
• Protein ID: WP_200921600.1
• Coordinates: 2486064..2486480 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS11900	2482618..2482848	-	231	WP_003020233.1	DUF2554 family protein	-
JLF47_RS11905	2483038..2483163	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
JLF47_RS11910	2483163..2484173	-	1011	WP_200921557.1	cytochrome d ubiquinol oxidase subunit II	-
JLF47_RS11915	2484173..2485576	-	1404	WP_164562763.1	cytochrome ubiquinol oxidase subunit I	-
JLF47_RS11920	2485841..2486038	+	198	WP_200921558.1	type II toxin-antitoxin system HicA family toxin	Toxin
JLF47_RS11925	2486064..2486480	+	417	WP_200921600.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
JLF47_RS11930	2486573..2487967	+	1395	WP_200932070.1	PLP-dependent aminotransferase family protein	-
JLF47_RS11935	2488295..2489440	+	1146	WP_008784842.1	ABC transporter substrate-binding protein	-
JLF47_RS11940	2489457..2490473	+	1017	WP_063940776.1	ABC transporter ATP-binding protein	-
JLF47_RS11945	2490474..2491418	+	945	WP_126957589.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7440.58 Da
• Isoelectric Point: 10.9523

>T35806 WP_200921558.1 NZ_AP022494:2485841-2486038 [Citrobacter portucalensis]
VKQSEFRRWLESQGVDVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLGLQCSLPVQG

Nucleotide

• Length: 198 bp

>T35806 NZ_AP022494:2485841-2486038 [Citrobacter portucalensis]
GTGAAGCAAAGTGAGTTCAGACGCTGGCTTGAATCTCAGGGAGTCGATGTGACAAATGGCTCAAATCATCTGAAGTTGAG
GTATCAGGGAAGGCGTAGCGTTATGCCCAGGCATCCCGGTGATGAAATAAAAGAGGCCTTGCGAAAAGCGATCATTAAGC
AGCTTGGTTTACAGTGTTCATTACCAGTACAAGGTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15092.26 Da
• Isoelectric Point: 4.3529

>AT35806 WP_200921600.1 NZ_AP022494:2486064-2486480 [Citrobacter portucalensis]
MRYPVNLIPAVEGGFVVSFPDIPEALTQGETRHEALQAAQGALVTAFEFYFDDNEPIPLPSAVGTEADYVEIPLSVASKV
LLLNAFLESNITQQELANRIGRPKQEITRLFDLNHATKIDAVQIAARALGKELSLTML

Nucleotide

• Length: 417 bp

>AT35806 NZ_AP022494:2486064-2486480 [Citrobacter portucalensis]
ATGCGTTATCCCGTTAATCTAATACCCGCCGTAGAAGGTGGATTTGTGGTTTCTTTCCCGGACATTCCTGAAGCATTGAC
TCAGGGAGAAACTCGCCATGAGGCTTTGCAAGCTGCACAGGGTGCATTGGTTACCGCTTTTGAATTCTATTTCGACGATA
ACGAACCGATTCCTTTACCTTCTGCAGTAGGTACTGAAGCTGACTATGTGGAAATACCACTTAGCGTTGCGTCTAAAGTT
CTGTTGTTAAATGCCTTTCTTGAATCCAATATCACCCAGCAGGAACTGGCAAACAGGATCGGCAGGCCTAAGCAGGAGAT
CACTCGCTTATTCGATTTAAATCACGCGACAAAAATCGATGCAGTGCAGATAGCTGCGCGCGCCTTAGGCAAAGAACTGT
CATTAACGATGTTGTAA