Detailed information of TA system TA035795

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 698074..698753
• Replicon: chromosome
• Accession: NZ_AP022494
• Organism: Citrobacter portucalensis strain STN0717-36

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7I6XXA8
• Locus tag: JLF47_RS03430
• Protein ID: WP_039025449.1
• Coordinates: 698412..698753 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF47_RS03425
• Protein ID: WP_200922882.1
• Coordinates: 698074..698391 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF47_RS03385	693112..693420	+	309	WP_185666326.1	monooxygenase	-
JLF47_RS03390	693557..694429	-	873	WP_200922887.1	alpha/beta hydrolase	-
JLF47_RS03395	694806..695045	+	240	WP_200922886.1	YceI family protein	-
JLF47_RS03405	696668..696859	+	192	Protein_668	DUF905 family protein	-
JLF47_RS03410	696873..697334	+	462	WP_200922885.1	antirestriction protein	-
JLF47_RS03415	697343..697825	+	483	WP_200922884.1	DNA repair protein RadC	-
JLF47_RS03420	697834..698055	+	222	WP_200922883.1	DUF987 domain-containing protein	-
JLF47_RS03425	698074..698391	+	318	WP_200922882.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF47_RS03430	698412..698753	+	342	WP_039025449.1	TA system toxin CbtA family protein	Toxin
JLF47_RS03440	699116..699622	+	507	WP_149281949.1	G/U mismatch-specific DNA glycosylase	-
JLF47_RS03445	699668..701515	-	1848	WP_003024699.1	RNA polymerase sigma factor RpoD	-
JLF47_RS03450	701875..703620	-	1746	WP_200921149.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	666966..703620	36654

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12880.79 Da
• Isoelectric Point: 9.6548

>T35795 WP_039025449.1 NZ_AP022494:698412-698753 [Citrobacter portucalensis]
MNTLPATTQRAAKPCLSPVAVWQMLLTRLLEKHYGLTLNDTPFSDETVIREHINAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNSVQ

Nucleotide

• Length: 342 bp

>T35795 NZ_AP022494:698412-698753 [Citrobacter portucalensis]
ATGAATACTTTACCTGCAACAACCCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGCTGCTGAC
CCGACTGCTCGAAAAGCACTATGGTCTGACCCTCAACGACACCCCGTTCAGCGATGAAACGGTTATTCGGGAACATATTA
ATGCTGGTATTACACTGGCTGATGCAGTGAATTTTCTGGTGGAAAAATACGAACTGGTCCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCACCTTATCTTCGGGCTGTAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAATTCGGTACAGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11854.56 Da
• Isoelectric Point: 6.9368

>AT35795 WP_200922882.1 NZ_AP022494:698074-698391 [Citrobacter portucalensis]
MSNITWGLQRAIMPCLGARLVQEGNRLHYLADRASITGKFSDAECRKLDETFPHFIRQMESMLTTGEVSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT35795 NZ_AP022494:698074-698391 [Citrobacter portucalensis]
ATGAGCAATATCACATGGGGCCTGCAGCGGGCTATCATGCCGTGCCTGGGAGCTCGTCTGGTGCAGGAGGGTAACCGACT
GCACTACCTAGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACGCCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAAGTCAGCCCCCAACATGCCCACTGCGTCATCCTGTACCAC
AACGGTTTCACCTGTGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA

Structures

Toxin

• AlphaFold DB: A0A7I6XXA8