Detailed information of TA system TA035791

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4800768..4801384
• Replicon: chromosome
• Accession: NZ_AP022486
• Organism: Citrobacter portucalensis strain STN0717-27

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLF36_RS22885
• Protein ID: WP_126957449.1
• Coordinates: 4800768..4801142 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7I7DRJ8
• Locus tag: JLF36_RS22890
• Protein ID: WP_003825279.1
• Coordinates: 4801142..4801384 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF36_RS22870	4798271..4799173	+	903	WP_003825273.1	formate dehydrogenase O subunit beta	-
JLF36_RS22875	4799170..4799805	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
JLF36_RS22880	4799802..4800731	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
JLF36_RS22885	4800768..4801142	-	375	WP_126957449.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF36_RS22890	4801142..4801384	-	243	WP_003825279.1	ribbon-helix-helix domain-containing protein	Antitoxin
JLF36_RS22895	4801590..4802498	+	909	WP_200920942.1	alpha/beta hydrolase	-
JLF36_RS22900	4802563..4802805	+	243	WP_003825282.1	type II toxin-antitoxin system ParD family antitoxin	-
JLF36_RS22905	4802798..4803085	+	288	WP_003825284.1	type II toxin-antitoxin system RelE/ParE family toxin	-
JLF36_RS22910	4803091..4804032	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
JLF36_RS22915	4804077..4804514	-	438	WP_126957455.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF36_RS22920	4804511..4805383	-	873	WP_008786998.1	virulence factor BrkB family protein	-
JLF36_RS22925	4805377..4805976	-	600	WP_003825293.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13682.87 Da
• Isoelectric Point: 9.6927

>T35791 WP_126957449.1 NZ_AP022486:c4801142-4800768 [Citrobacter portucalensis]
MVKGSALFDTNILIDLFSGRNEAKLAIETWSPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVISISQDIAERSVSI
RQEHGMKLPDAIILATAQLHRLTLVTRNTKDFAGISGVVTPYTL

Nucleotide

• Length: 375 bp

>T35791 NZ_AP022486:c4801142-4800768 [Citrobacter portucalensis]
ATGGTCAAAGGATCAGCGCTTTTTGATACCAATATCCTAATCGACCTGTTTAGCGGGAGAAATGAAGCAAAACTAGCGAT
AGAAACATGGTCGCCACAGAACGCAATCAGCCTCATTACATGGATGGAGGTAATGGTGGGCGCAAAAAAATACCATCAGG
AAGCCCGCACTCGCGTTGCGCTAGGTGCTTTTAACGTGATCAGCATTTCACAGGATATCGCTGAACGAAGCGTGAGTATC
AGGCAGGAACATGGGATGAAATTGCCGGATGCCATTATTTTGGCCACTGCGCAGCTTCATCGTCTTACACTGGTTACGCG
TAATACCAAGGATTTTGCAGGCATATCTGGCGTGGTCACGCCGTATACGTTATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9421.72 Da
• Isoelectric Point: 4.3437

>AT35791 WP_003825279.1 NZ_AP022486:c4801384-4801142 [Citrobacter portucalensis]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETAISRALGLWQGCEEDGVEYQRKLREEW

Nucleotide

• Length: 243 bp

>AT35791 NZ_AP022486:c4801384-4801142 [Citrobacter portucalensis]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGATGAGGTGATTAAACGTCTTGATGACCTCAAGGT
TCAGCGCAACCTGCCGCGAGCAGAACTCTTACGGGAGGCCGTTGAACAGTATCTTGAGAGGCAAGATCAGGCTGAAACAG
CTATTTCACGTGCTCTTGGGCTGTGGCAAGGCTGTGAGGAAGATGGTGTTGAATACCAGCGTAAACTACGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A7I7DRJ8