Detailed information of TA system TA035790

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4645983..4646571
• Replicon: chromosome
• Accession: NZ_AP022486
• Organism: Citrobacter portucalensis strain STN0717-27

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF36_RS22180
• Protein ID: WP_063941209.1
• Coordinates: 4645983..4646300 (+)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A5B0STC7
• Locus tag: JLF36_RS22185
• Protein ID: WP_032942057.1
• Coordinates: 4646293..4646571 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF36_RS22155	4641751..4642575	+	825	WP_200920925.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JLF36_RS22160	4642644..4643867	+	1224	WP_200920926.1	zinc-binding dehydrogenase	-
JLF36_RS22165	4643869..4644681	+	813	WP_063941212.1	shikimate 5-dehydrogenase	-
JLF36_RS22170	4644731..4645036	-	306	WP_032934126.1	hypothetical protein	-
JLF36_RS22175	4645168..4645719	+	552	WP_200920927.1	AAA family ATPase	-
JLF36_RS22180	4645983..4646300	+	318	WP_063941209.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF36_RS22185	4646293..4646571	+	279	WP_032942057.1	putative addiction module antidote protein	Antitoxin
JLF36_RS22190	4646682..4647371	+	690	WP_200921622.1	dipeptidase PepE	-
JLF36_RS22195	4647486..4649117	-	1632	WP_003826514.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4635973..4646571	10598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (3-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.09 Da
• Isoelectric Point: 10.1413

>T35790 WP_063941209.1 NZ_AP022486:4645983-4646300 [Citrobacter portucalensis]
IMEILQTMVFRRWEQNLRDRRAKTLIATRLFRLANGLSGDVKPVGEGVSEMRISYGPGYRIYFKQQGCRIIILLCGGDKS
SQANDITLAKMLARSLDYQEVFRHE

Nucleotide

• Length: 318 bp

>T35790 NZ_AP022486:4645983-4646300 [Citrobacter portucalensis]
ATCATGGAAATTCTTCAGACAATGGTTTTCCGGCGTTGGGAGCAAAACCTACGGGACAGGAGAGCTAAAACGCTCATTGC
CACACGCCTTTTTCGTTTGGCTAATGGTTTATCTGGTGATGTAAAACCTGTTGGGGAAGGCGTCAGCGAAATGCGCATTT
CTTACGGTCCTGGCTACAGGATTTACTTTAAACAGCAAGGCTGCCGCATCATCATTTTGTTATGTGGGGGCGATAAAAGC
TCACAAGCCAACGATATTACTCTTGCAAAAATGCTTGCCCGTTCACTGGATTATCAGGAGGTCTTCCGCCATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9872.45 Da
• Isoelectric Point: 5.8233

>AT35790 WP_032942057.1 NZ_AP022486:4646293-4646571 [Citrobacter portucalensis]
MNKLTHYDPATALVDDEEIAVFMADALETGDAAYIAKALGVVARAKGMANIAAETGLSREQLYRSFSEKGNPTLKTTLAV
MKVLGIELTLKK

Nucleotide

• Length: 279 bp

>AT35790 NZ_AP022486:4646293-4646571 [Citrobacter portucalensis]
ATGAATAAACTTACTCACTACGATCCCGCGACTGCGCTGGTTGATGATGAAGAAATTGCCGTGTTTATGGCCGATGCCCT
CGAAACAGGTGATGCAGCATACATTGCAAAAGCACTGGGCGTGGTGGCTCGTGCTAAGGGTATGGCGAACATCGCAGCAG
AAACGGGATTATCCCGCGAGCAGCTTTATCGCTCTTTTAGTGAGAAAGGTAACCCGACGCTGAAAACCACACTTGCGGTG
ATGAAGGTGCTCGGTATTGAATTGACACTTAAAAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A5B0STC7