Detailed information of TA system TA035787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2589161..2589897
• Replicon: chromosome
• Accession: NZ_AP022486
• Organism: Citrobacter portucalensis strain STN0717-27

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: JLF36_RS12415
• Protein ID: WP_200920428.1
• Coordinates: 2589415..2589897 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: JLF36_RS12410
• Protein ID: WP_008784769.1
• Coordinates: 2589161..2589427 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF36_RS12390	2584213..2585400	+	1188	WP_200920425.1	outer membrane protein transport protein	-
JLF36_RS12395	2585638..2586267	+	630	WP_003832974.1	winged helix-turn-helix transcriptional regulator	-
JLF36_RS12400	2586384..2586770	-	387	WP_200920426.1	hypothetical protein	-
JLF36_RS12405	2587025..2589007	+	1983	WP_200920427.1	MBL fold metallo-hydrolase	-
JLF36_RS12410	2589161..2589427	+	267	WP_008784769.1	DUF1778 domain-containing protein	Antitoxin
JLF36_RS12415	2589415..2589897	+	483	WP_200920428.1	GNAT family N-acetyltransferase	Toxin
JLF36_RS12420	2590100..2591503	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF36_RS12425	2591532..2592164	-	633	WP_001567369.1	hypothetical protein	-
JLF36_RS12430	2592545..2592661	-	117	Protein_2441	mechanosensitive ion channel protein MscS	-
JLF36_RS12435	2593093..2593470	+	378	WP_003832962.1	hypothetical protein	-
JLF36_RS12440	2593523..2594359	+	837	WP_200920429.1	DMT family transporter	-
JLF36_RS12445	2594369..2594890	-	522	WP_191222609.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2586384..2594890	8506
	-	flank	IS/Tn	-	-	2590100..2591503	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17625.46 Da
• Isoelectric Point: 9.5814

>T35787 WP_200920428.1 NZ_AP022486:2589415-2589897 [Citrobacter portucalensis]
VGFVTAPEPLTHIHQLAEFVSGEIVLDDWLKQKGLKNQALGAARTFVVCKTDTRQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSVRGKGLGADLLHDAVLRCYKVAENIGVRAVMVHALIDEAKRFYLHHGFKASQLQERTLFLRLS

Nucleotide

• Length: 483 bp

>T35787 NZ_AP022486:2589415-2589897 [Citrobacter portucalensis]
GTGGGATTTGTAACCGCACCGGAACCACTAACTCACATTCATCAATTAGCGGAATTTGTCAGCGGAGAAATTGTTCTCGA
TGATTGGTTAAAGCAAAAAGGGCTAAAAAATCAGGCTCTCGGCGCAGCCAGGACGTTTGTTGTCTGCAAAACAGACACGC
GACAAGTCGCTGGCTTTTATTCACTGGCCACAGGTAGCGTCAACCACACAGAAGCAACAGGCAATCTTCGGCGTAACATG
CCAGATCCAATCCCCGTAATTATCCTTGCCAGATTGGCCGTAGATCTTTCTGTTCGTGGAAAAGGGTTAGGGGCCGATCT
GCTTCACGATGCGGTACTGCGGTGTTACAAAGTGGCAGAAAATATTGGCGTGCGTGCCGTTATGGTACATGCGCTCATAG
ACGAAGCTAAACGTTTTTATCTTCACCACGGATTCAAAGCATCTCAACTGCAGGAACGAACTCTGTTCCTCAGGCTTTCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10159.65 Da
• Isoelectric Point: 4.6558

>AT35787 WP_008784769.1 NZ_AP022486:2589161-2589427 [Citrobacter portucalensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIEMLDAPVAEDPAIRKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT35787 NZ_AP022486:2589161-2589427 [Citrobacter portucalensis]
ATGAAATCAGATGTCCAGCTAAACCTTAGAGCTAAAGAATCCCAACGCGCGCTTATTGATGCGGCCGCAGAGATTCTACA
TAAATCGCGTACAGACTTTATTCTGGAAATGGCCTGCCAGGCGGCAGAGAACGTCATTCTCGATCGCCGAGTGTTTAATT
TTAATGATGAACAATACGCTGAGTTTATCGAGATGCTTGATGCTCCGGTTGCCGAAGATCCCGCTATCAGGAAGCTACTG
GCAAGGAAACCACAGTGGGATTTGTAA