Detailed information of TA system TA035774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 4858..5359
• Replicon: plasmid pSTN0717-20-2
• Accession: NZ_AP022484
• Organism: Escherichia coli strain STN0717-20

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: A0A6X6XTU7
• Locus tag: JLE48_RS23580
• Protein ID: WP_000702245.1
• Coordinates: 4858..5118 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: JLE48_RS23585
• Protein ID: WP_001132407.1
• Coordinates: 5108..5359 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE48_RS23540	457..618	+	162	WP_001366886.1	type I toxin-antitoxin system Hok family toxin	-
JLE48_RS23545	768..1364	+	597	WP_000125165.1	hypothetical protein	-
JLE48_RS23550	1382..1666	-	285	WP_001162371.1	hypothetical protein	-
JLE48_RS23555	1740..2405	-	666	WP_000532148.1	P-loop NTPase	-
JLE48_RS23560	3001..3330	+	330	WP_000855121.1	hypothetical protein	-
JLE48_RS23565	3911..4234	+	324	WP_000427402.1	hypothetical protein	-
JLE48_RS23570	4275..4484	+	210	WP_000866037.1	hypothetical protein	-
JLE48_RS23575	4582..4806	+	225	WP_000814383.1	hypothetical protein	-
JLE48_RS23580	4858..5118	+	261	WP_000702245.1	hypothetical protein	Toxin
JLE48_RS23585	5108..5359	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
JLE48_RS23590	5909..6187	+	279	WP_000545937.1	hypothetical protein	-
JLE48_RS23595	6574..6756	-	183	WP_001401700.1	hypothetical protein	-
JLE48_RS23600	7314..7496	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
JLE48_RS23605	7521..7958	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
JLE48_RS23610	8088..8408	-	321	WP_001401701.1	hypothetical protein	-
JLE48_RS23615	8742..8920	-	179	Protein_15	hypothetical protein	-
JLE48_RS23620	8922..9125	-	204	WP_000224735.1	hypothetical protein	-
JLE48_RS23625	9131..9568	-	438	WP_000206706.1	hypothetical protein	-
JLE48_RS23630	9588..10295	-	708	WP_000108795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..63027	63027

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9818.23 Da
• Isoelectric Point: 9.5163

>T35774 WP_000702245.1 NZ_AP022484:4858-5118 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEEAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T35774 NZ_AP022484:4858-5118 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCTGATCTTGTTTTTCATGAGGAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT35774 WP_001132407.1 NZ_AP022484:5108-5359 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT35774 NZ_AP022484:5108-5359 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCTGAAAT
ATTCAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A6X6XTU7