Detailed information of TA system TA035763

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4082806..4083638
• Replicon: chromosome
• Accession: NZ_AP022482
• Organism: Escherichia coli strain STN0717-20

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0Q2Y5N3
• Locus tag: JLE48_RS19505
• Protein ID: WP_001514886.1
• Coordinates: 4082806..4083180 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A7U9IW59
• Locus tag: JLE48_RS19510
• Protein ID: WP_001360327.1
• Coordinates: 4083270..4083638 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE48_RS19485	4080531..4080689	-	159	WP_001467148.1	hypothetical protein	-
JLE48_RS19490	4080789..4080965	-	177	WP_000839290.1	DUF957 domain-containing protein	-
JLE48_RS19495	4080982..4081344	-	363	Protein_3815	hypothetical protein	-
JLE48_RS19500	4081438..4082666	+	1229	WP_155105063.1	IS3 family transposase	-
JLE48_RS19505	4082806..4083180	-	375	WP_001514886.1	TA system toxin CbtA family protein	Toxin
JLE48_RS19510	4083270..4083638	-	369	WP_001360327.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLE48_RS19515	4083801..4084022	-	222	WP_000692286.1	DUF987 domain-containing protein	-
JLE48_RS19520	4084085..4084291	-	207	Protein_3820	hypothetical protein	-
JLE48_RS19525	4084376..4084801	+	426	WP_000422743.1	IS66 family insertion sequence hypothetical protein	-
JLE48_RS19530	4084798..4085148	+	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
JLE48_RS19535	4085179..4086792	+	1614	WP_000080197.1	IS66-like element ISEc23 family transposase	-
JLE48_RS19540	4086823..4087101	-	279	Protein_3824	RadC family protein	-
JLE48_RS19545	4087117..4087590	-	474	WP_000855059.1	antirestriction protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4081438..4086792	5354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13930.91 Da
• Isoelectric Point: 7.2909

>T35763 WP_001514886.1 NZ_AP022482:c4083180-4082806 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T35763 NZ_AP022482:c4083180-4082806 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.34 Da
• Isoelectric Point: 5.9598

>AT35763 WP_001360327.1 NZ_AP022482:c4083638-4083270 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT35763 NZ_AP022482:c4083638-4083270 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0Q2Y5N3

Antitoxin

• AlphaFold DB: A0A7U9IW59