Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2400173..2400811 Replicon chromosome
Accession NZ_AP022482
Organism Escherichia coli strain STN0717-20

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag JLE48_RS11405 Protein ID WP_000813794.1
Coordinates 2400635..2400811 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag JLE48_RS11400 Protein ID WP_001270286.1
Coordinates 2400173..2400589 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLE48_RS11375 2395490..2396143 - 654 Protein_2224 ABC transporter ATP-binding protein -
JLE48_RS11380 2396159..2396856 - 698 Protein_2225 IS1 family transposase -
JLE48_RS11385 2396912..2397280 - 369 Protein_2226 ABC transporter ATP-binding protein -
JLE48_RS11390 2397298..2398443 - 1146 WP_000047456.1 ABC transporter substrate-binding protein -
JLE48_RS11395 2398688..2400094 - 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
JLE48_RS11400 2400173..2400589 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
JLE48_RS11405 2400635..2400811 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
JLE48_RS11410 2401033..2401263 + 231 WP_000494244.1 YncJ family protein -
JLE48_RS11415 2401355..2403316 - 1962 WP_001307191.1 23S rRNA 5-hydroxycytidine C2501 synthase -
JLE48_RS11420 2403389..2403925 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
JLE48_RS11425 2403978..2405192 + 1215 WP_001349372.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2405232..2406497 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T35757 WP_000813794.1 NZ_AP022482:c2400811-2400635 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T35757 NZ_AP022482:c2400811-2400635 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT35757 WP_001270286.1 NZ_AP022482:c2400589-2400173 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT35757 NZ_AP022482:c2400589-2400173 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References