Detailed information of TA system TA035745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1314985..1315610
• Replicon: chromosome
• Accession: NZ_AP022482
• Organism: Escherichia coli strain STN0717-20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: JLE48_RS06350
• Protein ID: WP_000911330.1
• Coordinates: 1315212..1315610 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: JLE48_RS06345
• Protein ID: WP_000450524.1
• Coordinates: 1314985..1315212 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLE48_RS06320	1310788..1311258	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
JLE48_RS06325	1311258..1311830	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
JLE48_RS06330	1311976..1312854	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
JLE48_RS06335	1312871..1313905	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
JLE48_RS06340	1314118..1314831	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
JLE48_RS06345	1314985..1315212	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
JLE48_RS06350	1315212..1315610	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
JLE48_RS06355	1315757..1316620	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
JLE48_RS06360	1316635..1318650	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
JLE48_RS06365	1318724..1319422	+	699	WP_000679823.1	esterase	-
JLE48_RS06370	1319532..1319732	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T35745 WP_000911330.1 NZ_AP022482:1315212-1315610 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T35745 NZ_AP022482:1315212-1315610 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT35745 WP_000450524.1 NZ_AP022482:1314985-1315212 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT35745 NZ_AP022482:1314985-1315212 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33