Detailed information of TA system TA035740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 73832..74426
• Replicon: plasmid pSTN0717-11-1
• Accession: NZ_AP022479
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: JLF30_RS24475
• Protein ID: WP_001403920.1
• Coordinates: 73832..74107 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: JLF30_RS24480
• Protein ID: WP_001261274.1
• Coordinates: 74196..74426 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS24440	68913..69220	+	308	Protein_82	type II toxin-antitoxin system ParD family antitoxin	-
JLF30_RS24445	69563..69745	+	183	WP_042004909.1	hypothetical protein	-
JLF30_RS24450	69866..70606	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
JLF30_RS24455	70929..71918	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
JLF30_RS24460	72806..73075	+	270	WP_001275013.1	DUF1778 domain-containing protein	-
JLF30_RS24465	73079..73609	+	531	WP_000348883.1	GNAT family N-acetyltransferase	-
JLF30_RS24470	73610..73827	+	218	Protein_88	transposase	-
JLF30_RS24475	73832..74107	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF30_RS24480	74196..74426	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLF30_RS24485	74625..76799	-	2175	Protein_91	histidinol-phosphatase	-
JLF30_RS24490	76864..77619	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-
JLF30_RS24495	77616..79115	-	1500	WP_000174402.1	IS21-like element ISEc10 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5	-	1..91989	91989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T35740 WP_001403920.1 NZ_AP022479:c74107-73832 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T35740 NZ_AP022479:c74107-73832 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT35740 WP_001261274.1 NZ_AP022479:c74426-74196 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT35740 NZ_AP022479:c74426-74196 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1