Detailed information of TA system TA035739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 72806..73609
• Replicon: plasmid pSTN0717-11-1
• Accession: NZ_AP022479
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: JLF30_RS24465
• Protein ID: WP_000348883.1
• Coordinates: 73079..73609 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: JLF30_RS24460
• Protein ID: WP_001275013.1
• Coordinates: 72806..73075 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS24440	68913..69220	+	308	Protein_82	type II toxin-antitoxin system ParD family antitoxin	-
JLF30_RS24445	69563..69745	+	183	WP_042004909.1	hypothetical protein	-
JLF30_RS24450	69866..70606	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
JLF30_RS24455	70929..71918	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
JLF30_RS24460	72806..73075	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
JLF30_RS24465	73079..73609	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
JLF30_RS24470	73610..73827	+	218	Protein_88	transposase	-
JLF30_RS24475	73832..74107	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
JLF30_RS24480	74196..74426	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
JLF30_RS24485	74625..76799	-	2175	Protein_91	histidinol-phosphatase	-
JLF30_RS24490	76864..77619	-	756	WP_000065240.1	IS21-like element ISEc10 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5	-	1..91989	91989

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T35739 WP_000348883.1 NZ_AP022479:73079-73609 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T35739 NZ_AP022479:73079-73609 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT35739 WP_001275013.1 NZ_AP022479:72806-73075 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT35739 NZ_AP022479:72806-73075 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7