35738

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 66097..66656 Replicon plasmid pSTN0717-11-1
Accession NZ_AP022479
Organism Escherichia coli strain STN0717-11

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag JLF30_RS24420 Protein ID WP_000421272.1
Coordinates 66381..66656 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag JLF30_RS24415 Protein ID WP_001387467.1
Coordinates 66097..66381 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF30_RS24390 61560..62000 - 441 Protein_72 zinc-binding dehydrogenase -
JLF30_RS24395 62401..63291 + 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
JLF30_RS24400 63316..63696 + 381 WP_000710783.1 Rid family detoxifying hydrolase -
JLF30_RS24405 63729..64694 + 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
JLF30_RS24410 64740..65492 - 753 WP_000562172.1 IclR family transcriptional regulator -
JLF30_RS24415 66097..66381 + 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
JLF30_RS24420 66381..66656 + 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF30_RS24425 66762..67055 + 294 WP_001105060.1 hypothetical protein -
JLF30_RS24430 67090..67218 - 129 Protein_80 IS481 family transposase -
JLF30_RS24435 67251..68420 - 1170 WP_001072355.1 hypothetical protein -
JLF30_RS24440 68913..69220 + 308 Protein_82 type II toxin-antitoxin system ParD family antitoxin -
JLF30_RS24445 69563..69745 + 183 WP_042004909.1 hypothetical protein -
JLF30_RS24450 69866..70606 + 741 WP_001586233.1 tyrosine-type recombinase/integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 - 1..91989 91989


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T35738 WP_000421272.1 NZ_AP022479:66381-66656 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T35738 NZ_AP022479:66381-66656 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT35738 WP_001387467.1 NZ_AP022479:66097-66381 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT35738 NZ_AP022479:66097-66381 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References