Detailed information of TA system TA035736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4487272..4487867
• Replicon: chromosome
• Accession: NZ_AP022478
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: JLF30_RS21335
• Protein ID: WP_000239581.1
• Coordinates: 4487272..4487622 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: JLF30_RS21340
• Protein ID: WP_001223213.1
• Coordinates: 4487616..4487867 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS21315	4482719..4483741	-	1023	WP_001586590.1	ABC transporter permease	-
JLF30_RS21320	4483755..4485257	-	1503	WP_000210560.1	sugar ABC transporter ATP-binding protein	-
JLF30_RS21325	4485397..4486353	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
JLF30_RS21330	4486663..4487193	+	531	WP_000055075.1	inorganic diphosphatase	-
JLF30_RS21335	4487272..4487622	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
JLF30_RS21340	4487616..4487867	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
JLF30_RS21345	4488079..4488420	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
JLF30_RS21350	4488423..4492202	-	3780	WP_001586589.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T35736 WP_000239581.1 NZ_AP022478:c4487622-4487272 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T35736 NZ_AP022478:c4487622-4487272 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT35736 WP_001223213.1 NZ_AP022478:c4487867-4487616 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT35736 NZ_AP022478:c4487867-4487616 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7