Detailed information of TA system TA035730

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 3919996..3920690
• Replicon: chromosome
• Accession: NZ_AP022478
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A1X9TDN4
• Locus tag: JLF30_RS18680
• Protein ID: WP_001094398.1
• Coordinates: 3919996..3920364 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: JLF30_RS18685
• Protein ID: WP_077249034.1
• Coordinates: 3920385..3920690 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS18650	3915910..3916011	+	102	WP_000662257.1	hypothetical protein	-
JLF30_RS18655	3916376..3916639	+	264	WP_000803998.1	type B 50S ribosomal protein L31	-
JLF30_RS18660	3916639..3916779	+	141	WP_000866436.1	type B 50S ribosomal protein L36	-
JLF30_RS18665	3916849..3917039	-	191	Protein_3647	hypothetical protein	-
JLF30_RS18670	3917826..3918523	+	698	Protein_3648	IS1 family transposase	-
JLF30_RS18675	3918516..3919222	+	707	Protein_3649	integrase domain-containing protein	-
JLF30_RS18680	3919996..3920364	-	369	WP_001094398.1	TA system toxin CbtA family protein	Toxin
JLF30_RS18685	3920385..3920690	-	306	WP_077249034.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
JLF30_RS18690	3920794..3921438	-	645	WP_000086755.1	hypothetical protein	-
JLF30_RS18695	3921457..3921678	-	222	WP_000692345.1	DUF987 domain-containing protein	-
JLF30_RS18700	3921747..3922223	-	477	WP_001424026.1	RadC family protein	-
JLF30_RS18705	3922239..3922712	-	474	WP_000855059.1	antirestriction protein	-
JLF30_RS18710	3923054..3923875	-	822	WP_001234565.1	DUF945 domain-containing protein	-
JLF30_RS18715	3923976..3924184	-	209	Protein_3657	DUF905 family protein	-
JLF30_RS18720	3924285..3924740	-	456	WP_000581506.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC	3898601..3934339	35738

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (3-100)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13581.73 Da
• Isoelectric Point: 7.7465

>T35730 WP_001094398.1 NZ_AP022478:c3920364-3919996 [Escherichia coli]
MNTLPATISPAAKPCPSPVAVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
NSQVQAPYLTATDILQARKACGLMSRCSYRDVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T35730 NZ_AP022478:c3920364-3919996 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCTCCGGCGGCGAAGCCCTGTCCGTCGCCCGTTGCAGTCTGGCAAATGTTACTTAC
TCGTTTGCTTGAGCAGCACTATGGCCTAATGCTAAGCGACACACCTTTTAGCGATGAAACCGTTATAAAGGAGCATATTG
ATGCCGGTATCACTCTGGCTAATGCCGTTAACTTTCTGGTAGAGAAATATGAGTTAGTTCGTATTGATCGCAATGGATTT
AACTCTCAGGTACAAGCACCATATTTGACTGCGACAGACATTCTTCAAGCCAGGAAAGCGTGCGGGTTGATGAGCCGGTG
TTCTTACAGAGATGTAAGTAATATTGTTCTTAGCCGATCACGGTTGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11263.85 Da
• Isoelectric Point: 6.0454

>AT35730 WP_077249034.1 NZ_AP022478:c3920690-3920385 [Escherichia coli]
MVGLPYTVTPCSGTRLVQEGNRLYYLADRASITGKFSDAESLKLDVVFPHFISQMESMLTTGEMNPRHAHCFTLYHNGFT
CEADTLGSCGYVYIAIYPTQL

Nucleotide

• Length: 306 bp

>AT35730 NZ_AP022478:c3920690-3920385 [Escherichia coli]
CTGGTGGGGCTACCCTACACCGTGACGCCCTGTTCTGGCACCCGTCTGGTGCAGGAGGGCAACCGACTGTACTATTTGGC
TGACCGGGCAAGTATCACCGGTAAGTTTAGTGATGCCGAAAGCCTGAAGCTGGATGTCGTATTTCCACATTTTATCAGTC
AGATGGAATCGATGCTGACCACTGGTGAAATGAATCCCCGCCATGCCCACTGTTTCACCCTGTACCACAACGGTTTCACC
TGCGAAGCCGATACCCTTGGTAGTTGCGGCTATGTATACATCGCCATTTATCCCACTCAGCTCTAA

Structures

Toxin

• AlphaFold DB: A0A1X9TDN4