35729

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3737532..3738150 Replicon chromosome
Accession NZ_AP022478
Organism Escherichia coli strain STN0717-11

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag JLF30_RS17835 Protein ID WP_001291435.1
Coordinates 3737932..3738150 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag JLF30_RS17830 Protein ID WP_000344800.1
Coordinates 3737532..3737906 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF30_RS17820 3732621..3733814 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
JLF30_RS17825 3733837..3736986 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
JLF30_RS17830 3737532..3737906 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
JLF30_RS17835 3737932..3738150 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
JLF30_RS17840 3738322..3738873 + 552 WP_000102564.1 maltose O-acetyltransferase -
JLF30_RS17845 3738989..3739459 + 471 WP_000136192.1 YlaC family protein -
JLF30_RS17850 3739623..3741173 + 1551 WP_001305493.1 cyclic-guanylate-specific phosphodiesterase PdeB -
JLF30_RS17855 3741215..3741568 - 354 WP_000878140.1 DUF1428 domain-containing protein -
JLF30_RS17865 3741947..3742258 + 312 WP_000409911.1 MGMT family protein -
JLF30_RS17870 3742289..3742861 - 573 WP_000779833.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T35729 WP_001291435.1 NZ_AP022478:3737932-3738150 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T35729 NZ_AP022478:3737932-3738150 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT35729 WP_000344800.1 NZ_AP022478:3737532-3737906 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT35729 NZ_AP022478:3737532-3737906 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References