Detailed information of TA system TA035727

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 3040329..3041113
• Replicon: chromosome
• Accession: NZ_AP022478
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: JLF30_RS14565
• Protein ID: WP_000613626.1
• Coordinates: 3040619..3041113 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: JLF30_RS14560
• Protein ID: WP_001110447.1
• Coordinates: 3040329..3040622 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS14550	3035479..3036438	-	960	WP_001586906.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
JLF30_RS14555	3037011..3040196	+	3186	WP_001522867.1	ribonuclease E	-
JLF30_RS14560	3040329..3040622	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
JLF30_RS14565	3040619..3041113	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
JLF30_RS14570	3041208..3042161	-	954	WP_001586905.1	flagellar hook-associated protein FlgL	-
JLF30_RS14575	3042173..3043816	-	1644	WP_000096478.1	flagellar hook-associated protein FlgK	-
JLF30_RS14580	3043882..3044823	-	942	WP_001586904.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
JLF30_RS14585	3044823..3045920	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T35727 WP_000613626.1 NZ_AP022478:3040619-3041113 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T35727 NZ_AP022478:3040619-3041113 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT35727 WP_001110447.1 NZ_AP022478:3040329-3040622 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT35727 NZ_AP022478:3040329-3040622 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6