Detailed information of TA system TA035726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2611470..2612108
• Replicon: chromosome
• Accession: NZ_AP022478
• Organism: Escherichia coli strain STN0717-11

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D3GRG9
• Locus tag: JLF30_RS12260
• Protein ID: WP_001314712.1
• Coordinates: 2611932..2612108 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: JLF30_RS12255
• Protein ID: WP_077248684.1
• Coordinates: 2611470..2611886 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF30_RS12235	2606622..2607563	-	942	WP_001587036.1	ABC transporter permease	-
JLF30_RS12240	2607564..2608577	-	1014	WP_000220407.1	ABC transporter ATP-binding protein	-
JLF30_RS12245	2608595..2609740	-	1146	WP_000047443.1	ABC transporter substrate-binding protein	-
JLF30_RS12250	2609985..2611391	-	1407	WP_000760623.1	PLP-dependent aminotransferase family protein	-
JLF30_RS12255	2611470..2611886	-	417	WP_077248684.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
JLF30_RS12260	2611932..2612108	-	177	WP_001314712.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
JLF30_RS12265	2612330..2612560	+	231	WP_000494241.1	YncJ family protein	-
JLF30_RS12270	2612652..2614613	-	1962	WP_001587031.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
JLF30_RS12275	2614686..2615222	-	537	WP_000429149.1	DNA-binding transcriptional regulator SutR	-
JLF30_RS12280	2615275..2616486	+	1212	WP_071593600.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (87-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6746.81 Da
• Isoelectric Point: 11.8888

>T35726 WP_001314712.1 NZ_AP022478:c2612108-2611932 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T35726 NZ_AP022478:c2612108-2611932 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCAATGTAGCAAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTACGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.60 Da
• Isoelectric Point: 4.7286

>AT35726 WP_077248684.1 NZ_AP022478:c2611886-2611470 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT35726 NZ_AP022478:c2611886-2611470 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGACATCACTCAGCAAGAATTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTGA

Structures

Toxin

• AlphaFold DB: D3GRG9