Detailed information of TA system TA035709

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9082..9752
• Replicon: plasmid pSTW0522-72-3
• Accession: NZ_AP022476
• Organism: Pseudomonas monteilii strain STW0522-72

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q2QCR9
• Locus tag: JLF49_RS27445
• Protein ID: WP_012477632.1
• Coordinates: 9315..9752 (+)
• Length: 146 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q2QCS0
• Locus tag: JLF49_RS27440
• Protein ID: WP_012477631.1
• Coordinates: 9082..9318 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF49_RS27410	4322..4528	+	207	WP_015272391.1	zinc ribbon domain-containing protein	-
JLF49_RS27415	4521..5138	+	618	WP_015272392.1	recombinase family protein	-
JLF49_RS27420	5125..6261	+	1137	WP_015272393.1	replication initiator protein A	-
JLF49_RS27425	7021..7179	-	159	WP_200929578.1	hypothetical protein	-
JLF49_RS27430	7374..8024	+	651	WP_015272395.1	DNA-binding protein	-
JLF49_RS27435	8647..9072	+	426	WP_015272397.1	DUF192 domain-containing protein	-
JLF49_RS27440	9082..9318	+	237	WP_012477631.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
JLF49_RS27445	9315..9752	+	438	WP_012477632.1	type II toxin-antitoxin system VapC family toxin	Toxin
JLF49_RS27450	9759..9920	+	162	WP_015272398.1	hypothetical protein	-
JLF49_RS27455	10048..10308	+	261	WP_015272399.1	hypothetical protein	-
JLF49_RS27460	10989..11825	+	837	WP_015272400.1	DUF945 domain-containing protein	-
JLF49_RS27465	11881..12309	+	429	WP_015272401.1	hypothetical protein	-
JLF49_RS27470	12326..12829	+	504	WP_015272402.1	hypothetical protein	-
JLF49_RS27475	12890..13240	+	351	WP_015272403.1	hypothetical protein	-
JLF49_RS27480	13288..13500	+	213	WP_153334493.1	hypothetical protein	-
JLF49_RS27485	14158..14412	+	255	WP_015272405.1	hypothetical protein	-
JLF49_RS27490	14483..14659	+	177	WP_019692578.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-2 / blaIMP-11 / sul1 / qacE	-	1..44219	44219
	-	inside	Integron	-	-	69..43849	43780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 146 a.a.
• Molecular weight: 15671.07 Da
• Isoelectric Point: 8.5199

>T35709 WP_012477632.1 NZ_AP022476:9315-9752 [Pseudomonas monteilii]
MSKKTYMLDTNICSFIMRERPDAVLAKLEQAVTNQHRIVVSAITYSEMRFGAANPKASPKVAAMVDAFIQRLDAILSWDA
AAVDQTTQIRTALARLGTPIGNNDAAIAGHALAAGCVLVTNNTREFARVPGLVLEDWTQPAITKS

Nucleotide

• Length: 438 bp

>T35709 NZ_AP022476:9315-9752 [Pseudomonas monteilii]
ATGAGCAAAAAGACGTACATGCTCGATACGAACATCTGTTCGTTCATCATGCGCGAGCGCCCCGACGCGGTGCTGGCCAA
GCTGGAACAGGCAGTCACTAATCAGCACCGGATTGTCGTTTCGGCCATCACCTACTCCGAAATGCGTTTCGGGGCCGCCA
ACCCCAAGGCATCGCCCAAGGTTGCAGCGATGGTCGATGCCTTCATCCAGCGCCTGGACGCCATCCTGTCCTGGGACGCC
GCCGCCGTAGATCAAACAACGCAGATCCGCACCGCCCTCGCCCGCCTGGGTACGCCCATTGGCAACAACGATGCAGCGAT
AGCCGGCCATGCCCTGGCCGCTGGGTGTGTTCTGGTCACGAACAACACTCGCGAATTTGCTCGCGTTCCTGGCCTAGTCC
TTGAGGACTGGACCCAACCCGCCATTACCAAGAGTTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 9070.29 Da
• Isoelectric Point: 4.4563

>AT35709 WP_012477631.1 NZ_AP022476:9082-9318 [Pseudomonas monteilii]
MRTVSIFMNSRNQAIRIPRDMEYQGVSELEIIRDGETLILRPVRPSWASLRDVAAADPDFLTERKDVIEEDRFLGDFT

Nucleotide

• Length: 237 bp

>AT35709 NZ_AP022476:9082-9318 [Pseudomonas monteilii]
ATGCGTACCGTTTCCATTTTCATGAACAGCCGAAACCAGGCGATCCGCATCCCTCGGGATATGGAGTACCAGGGCGTTTC
CGAGCTGGAGATTATCCGCGACGGCGAAACGCTGATCCTGCGCCCTGTGCGCCCCTCCTGGGCATCGTTGCGCGACGTGG
CGGCCGCAGATCCTGACTTTCTGACCGAGCGAAAGGATGTGATCGAGGAAGACCGTTTCCTGGGTGACTTCACATGA

Structures

Toxin

• AlphaFold DB: A0A010RDD2

Antitoxin

• AlphaFold DB: A0A010SZE6