Detailed information of TA system TA035708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4699077..4699663
• Replicon: chromosome
• Accession: NZ_AP022473
• Organism: Pseudomonas monteilii strain STW0522-72

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF49_RS22260
• Protein ID: WP_082426425.1
• Coordinates: 4699370..4699663 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: JLF49_RS22255
• Protein ID: WP_182408666.1
• Coordinates: 4699077..4699373 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF49_RS22235	4696045..4696986	-	942	WP_200928792.1	FecR domain-containing protein	-
JLF49_RS22240	4696986..4697492	-	507	WP_060499947.1	sigma-70 family RNA polymerase sigma factor	-
JLF49_RS22245	4697788..4698435	+	648	WP_060499948.1	hypothetical protein	-
JLF49_RS22250	4698587..4699075	+	489	WP_104833975.1	GNAT family N-acetyltransferase	-
JLF49_RS22255	4699077..4699373	-	297	WP_182408666.1	putative addiction module antidote protein	Antitoxin
JLF49_RS22260	4699370..4699663	-	294	WP_082426425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF49_RS22265	4699885..4700772	+	888	WP_060499951.1	effector protein	-
JLF49_RS22270	4700769..4701536	-	768	WP_060499952.1	phosphonate ABC transporter, permease protein PhnE	-
JLF49_RS22275	4701533..4702360	-	828	WP_060499953.1	ABC transporter permease subunit	-
JLF49_RS22280	4702354..4703163	-	810	WP_182408667.1	ATP-binding cassette domain-containing protein	-
JLF49_RS22285	4703160..4704014	-	855	WP_060499955.1	putative selenate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10847.80 Da
• Isoelectric Point: 10.5184

>T35708 WP_082426425.1 NZ_AP022473:c4699663-4699370 [Pseudomonas monteilii]
MYLVEQTESFAVWLSSLRDLKAKLAIGRRLERVAIGNLGDFKWLGGGLGELRIDVGAGYRVYFTQKGHRLVLLLVGGDKS
TQVADILKSRKLLKEMI

Nucleotide

• Length: 294 bp

>T35708 NZ_AP022473:c4699663-4699370 [Pseudomonas monteilii]
ATGTATCTTGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGGGATTTGAAAGCCAAGCTTGCGATAGG
TCGCAGGCTGGAGCGAGTTGCGATAGGCAACCTTGGTGACTTCAAGTGGCTTGGAGGCGGGCTAGGTGAGCTTCGCATCG
ACGTAGGTGCAGGCTATCGCGTCTATTTCACGCAAAAGGGGCACAGATTGGTTCTGCTGCTGGTTGGCGGCGACAAGTCC
ACTCAAGTCGCCGATATTCTCAAATCAAGAAAGCTGCTGAAGGAGATGATATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10617.30 Da
• Isoelectric Point: 6.2205

>AT35708 WP_182408666.1 NZ_AP022473:c4699373-4699077 [Pseudomonas monteilii]
MSEKLIPFDMAALLSSDEAISEYLSQVLSDGDTEEIIRALGHIARARGMTHIAMESGLGRESLYKALSPGAKPRFDTVLK
VIRALGVDLLVQPHAVAR

Nucleotide

• Length: 297 bp

>AT35708 NZ_AP022473:c4699373-4699077 [Pseudomonas monteilii]
ATGAGCGAAAAACTGATTCCGTTTGATATGGCAGCTTTGTTGAGTAGCGATGAGGCCATCAGTGAGTACCTTAGCCAGGT
GCTATCAGATGGCGATACCGAAGAGATCATTCGAGCGCTCGGCCATATCGCGCGTGCACGAGGCATGACACACATAGCCA
TGGAGAGTGGTTTGGGCAGAGAAAGCCTCTACAAGGCGCTCAGCCCAGGCGCAAAGCCTCGTTTTGACACTGTGTTGAAG
GTCATTCGAGCCTTGGGGGTAGACCTTCTCGTGCAGCCCCATGCGGTAGCCCGTTGA