Detailed information of TA system TA035707

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4692727..4693335
• Replicon: chromosome
• Accession: NZ_AP022473
• Organism: Pseudomonas monteilii strain STW0522-72

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: JLF49_RS22225
• Protein ID: WP_200928790.1
• Coordinates: 4693045..4693335 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A6G6V4Q8
• Locus tag: JLF49_RS22220
• Protein ID: WP_060499944.1
• Coordinates: 4692727..4693035 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF49_RS22195	4687898..4689760	-	1863	WP_060501849.1	protein translocase subunit SecD	-
JLF49_RS22200	4689824..4690159	-	336	WP_008092587.1	preprotein translocase subunit YajC	-
JLF49_RS22205	4690202..4691317	-	1116	WP_014589748.1	tRNA guanosine(34) transglycosylase Tgt	-
JLF49_RS22210	4691332..4692381	-	1050	WP_104834019.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
JLF49_RS22220	4692727..4693035	-	309	WP_060499944.1	putative addiction module antidote protein	Antitoxin
JLF49_RS22225	4693045..4693335	-	291	WP_200928790.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLF49_RS22230	4693447..4695969	-	2523	WP_200928791.1	TonB-dependent receptor	-
JLF49_RS22235	4696045..4696986	-	942	WP_200928792.1	FecR domain-containing protein	-
JLF49_RS22240	4696986..4697492	-	507	WP_060499947.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (4-96)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10711.19 Da
• Isoelectric Point: 8.9632

>T35707 WP_200928790.1 NZ_AP022473:c4693335-4693045 [Pseudomonas monteilii]
VKTILTTDDFDAWFSKLSDKRSAMRIQARIDRAEAGNFGDCQAVGEGVSEMRIHYGPGYRVYFTQRGLEIVLLLAGGDKS
TQSKDIKTALDIARRL

Nucleotide

• Length: 291 bp

>T35707 NZ_AP022473:c4693335-4693045 [Pseudomonas monteilii]
GTGAAGACCATCCTCACAACCGATGATTTCGATGCTTGGTTTTCCAAGCTTAGCGATAAGCGATCTGCGATGCGCATCCA
GGCGCGCATCGACCGTGCCGAAGCAGGGAATTTCGGTGATTGCCAAGCTGTGGGTGAGGGCGTCTCCGAGATGCGTATTC
ACTATGGGCCAGGATATCGTGTGTACTTCACCCAGCGCGGCCTTGAAATCGTGCTCCTGCTGGCGGGTGGCGATAAGTCC
ACTCAATCAAAAGACATCAAAACCGCCCTCGACATTGCACGACGGCTTTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10817.32 Da
• Isoelectric Point: 6.2202

>AT35707 WP_060499944.1 NZ_AP022473:c4693035-4692727 [Pseudomonas monteilii]
MGNVKLKKWDSAEHLKTDEDMALYLEACIEEAGDDAAFIAKALGTIARAKGMAQLARDTGLGRESLYKALSGEGNPSFAT
ILKVTRALGLQLHADVASTHTG

Nucleotide

• Length: 309 bp

>AT35707 NZ_AP022473:c4693035-4692727 [Pseudomonas monteilii]
ATGGGTAACGTCAAGTTGAAAAAATGGGACAGTGCCGAACACCTCAAGACGGACGAGGACATGGCGCTTTACCTCGAAGC
TTGCATTGAAGAGGCGGGTGATGATGCGGCTTTTATCGCCAAGGCGCTCGGCACCATTGCGCGTGCAAAAGGCATGGCTC
AGTTGGCCCGTGATACCGGGCTTGGGCGTGAAAGCCTGTACAAGGCGTTGTCCGGCGAGGGGAACCCAAGCTTCGCCACC
ATCCTGAAGGTGACCAGAGCGCTTGGCCTACAGCTTCATGCTGACGTTGCATCCACTCATACTGGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A6G6V4Q8