Detailed information of TA system TA035705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 252340..253076
• Replicon: plasmid pSTW0522-66-1
• Accession: NZ_AP022466
• Organism: Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: JLF42_RS25310
• Protein ID: WP_003026803.1
• Coordinates: 252340..252822 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: JLF42_RS25315
• Protein ID: WP_003026799.1
• Coordinates: 252810..253076 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF42_RS25290	248059..248454	-	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
JLF42_RS25295	248503..249849	-	1347	WP_077253535.1	ISNCY family transposase	-
JLF42_RS25300	250075..250707	+	633	WP_001567369.1	hypothetical protein	-
JLF42_RS25305	250736..252139	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
JLF42_RS25310	252340..252822	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
JLF42_RS25315	252810..253076	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
JLF42_RS25320	253521..253751	-	231	WP_023307222.1	hypothetical protein	-
JLF42_RS25325	253765..253968	-	204	WP_004213596.1	hemolysin expression modulator Hha	-
JLF42_RS25330	254120..255088	+	969	WP_023307223.1	IS5-like element IS903B family transposase	-
JLF42_RS25335	255452..256192	+	741	WP_001515717.1	tyrosine-type recombinase/integrase	-
JLF42_RS25340	256890..257900	-	1011	WP_000200070.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mcr-10	-	1..324199	324199
	-	inside	IScluster/Tn	-	-	246473..255088	8615

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T35705 WP_003026803.1 NZ_AP022466:c252822-252340 [Enterobacter roggenkampii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T35705 NZ_AP022466:c252822-252340 [Enterobacter roggenkampii]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTGCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT35705 WP_003026799.1 NZ_AP022466:c253076-252810 [Enterobacter roggenkampii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT35705 NZ_AP022466:c253076-252810 [Enterobacter roggenkampii]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66