Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1102767..1103387 Replicon chromosome
Accession NZ_AP022465
Organism Enterobacter roggenkampii strain STW0522-66

Toxin (Protein)


Gene name Hha Uniprot ID A0A1H8SI38
Locus tag JLF42_RS05145 Protein ID WP_008499287.1
Coordinates 1102767..1102985 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag JLF42_RS05150 Protein ID WP_008499288.1
Coordinates 1103013..1103387 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF42_RS05115 1098780..1099040 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
JLF42_RS05120 1099043..1099183 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
JLF42_RS05125 1099180..1099890 - 711 WP_023343500.1 GNAT family N-acetyltransferase -
JLF42_RS05130 1099992..1101452 + 1461 WP_032664011.1 PLP-dependent aminotransferase family protein -
JLF42_RS05135 1101424..1101891 - 468 WP_008499285.1 membrane protein -
JLF42_RS05140 1102009..1102560 - 552 WP_055321802.1 maltose O-acetyltransferase -
JLF42_RS05145 1102767..1102985 - 219 WP_008499287.1 hemolysin expression modulator Hha Toxin
JLF42_RS05150 1103013..1103387 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
JLF42_RS05155 1103897..1107043 - 3147 WP_008499289.1 multidrug efflux RND transporter permease subunit -
JLF42_RS05160 1107066..1108259 - 1194 WP_021241633.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8612.00 Da        Isoelectric Point: 8.9008

>T35691 WP_008499287.1 NZ_AP022465:c1102985-1102767 [Enterobacter roggenkampii]
MSDKPLTKIDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFVR

Download         Length: 219 bp

>T35691 NZ_AP022465:c1102985-1102767 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACTAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT35691 WP_008499288.1 NZ_AP022465:c1103387-1103013 [Enterobacter roggenkampii]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT35691 NZ_AP022465:c1103387-1103013 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8SI38

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References